Gunji Y, Tagawa M, Matsubara H, Takenaga K, Shimada H, Kondo F, Suzuki T, Nakajima K, Sugaya M, Asano T, Ochiai T, Isono K, Kageyama H, Nakamura Y, Sakiyama S
Department of Surgery (II), Chiba University School of Medicine, Japan.
Oncology. 1997 Jan-Feb;54(1):69-73. doi: 10.1159/000227664.
We have investigated the antitumor effect of murine colon carcinoma cells developed to produce cytokines. Retrovirally transduced cells were inoculated into syngeneic mice, and their survivals were examined. Inoculation of interleukin-4 (IL-4)- or granulocyte macrophage-colony stimulating factor (GM-CSF)-producing cells alone did not affect the survival of the mice. In the tumors derived from an admixture of IL-4 and GM-CSF producers, however, regression developed spontaneously, and prolonged survival of the challenged mice was consequently observed. Histological examination revealed predominant infiltration of neutrophils around the tumor of a mixed population of IL-4 and GM-CSF producers.
我们研究了经基因改造以产生细胞因子的小鼠结肠癌细胞的抗肿瘤作用。将逆转录病毒转导的细胞接种到同基因小鼠体内，并检测它们的存活情况。单独接种产生白细胞介素-4（IL-4）或粒细胞巨噬细胞集落刺激因子（GM-CSF）的细胞对小鼠的存活没有影响。然而，在由产生IL-4和GM-CSF的细胞混合形成的肿瘤中，肿瘤会自发消退，因此观察到受攻击小鼠的存活期延长。组织学检查显示，在产生IL-4和GM-CSF的细胞混合群体的肿瘤周围，有大量中性粒细胞浸润。
