使用抗溴脱氧尿苷单克隆抗体分析γ射线照射后的细胞动力学。

Analysis of cell kinetics after gamma-ray irradiation using an anti-BrdU monoclonal antibody.

作者信息

Murata T, Akagi K, Nasu R, Kimura H, Tanaka Y

机构信息

Department of Radiology, Kansai Medical University, Osaka, Japan.

出版信息

Int J Oncol. 1998 Feb;12(2):345-9. doi: 10.3892/ijo.12.2.345.

DOI:10.3892/ijo.12.2.345

PMID:9458360

Abstract

The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrdU) monoclonal antibody and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied primarily by counting G2-arrested cells. By the present BrdU method, a rapid transition from G1 to S-phase was observed within 2 h of irradiation, followed by G1 block. Cells in the S-phase progressed to G2+M where they arrested, resulting in a decreased percentage of S cells (<5%). Release of G1 block occurred after 8 h, and G2+M cells returned to G1 after >18 h. The initial G1 arrest induced by irradiation was confirmed for the first time by the present BrdU-PI double staining.

摘要

使用抗溴脱氧尿苷（BrdU）单克隆抗体和碘化丙啶（PI）进行双重染色分析细胞周期。将照射后细胞动力学的变化与传统基于PI的DNA直方图方法所观察到的变化进行比较。主要通过计数G2期阻滞细胞来研究照射对细胞动力学的影响。通过目前的BrdU方法，在照射后2小时内观察到从G1期到S期的快速转变，随后出现G1期阻滞。S期细胞进展到G2+M期并在该期阻滞，导致S期细胞百分比降低（<5%）。G1期阻滞在8小时后解除，G2+M期细胞在>18小时后返回G1期。目前的BrdU-PI双重染色首次证实了照射诱导的初始G1期阻滞。