Castex N, Fioramonti J, Ducos de Lahitte J, Luffau G, More J, Bueno L
Department of Pharmacology, Institut National de la Recherche Agronomique, Toulouse, France.
Am J Physiol. 1998 Jan;274(1):G210-6. doi: 10.1152/ajpgi.1998.274.1.G210.
Brain-gut interactions and intestinal motility were studied during pulmonary and jejunal inflammation induced by Nippostrongylus brasiliensis. Jejunal electromyographic activity was continuously recorded from day 1 before to day 28 after infection. Expression of c-fos was assessed in the brain by immunohistochemistry, and myeloperoxidase (MPO) activity was determined in lung and intestine on days 1,7,14, 21, and 28 postinfection. The cyclic intestinal motor pattern was replaced by an irregular activity from day 4, corresponding to larvae migration to the intestine, to day 14. c-fos was expressed in the caudal nucleus of the solitary tract (NTS) and lateral parabrachial nucleus (LPB) on day 1 (lung stage of N. brasiliensis) and in the medial part of the NTS, the LPB, and locus ceruleus on day 7. Pulmonary and intestinal MPO activity was increased from days 1 to 21 postinfection. During N. brasiliensis infection, c-fos expression indicates that specific and different brain nuclei are activated at the onset of pulmonary and intestinal inflammation, which is associated with motor disorders.
在巴西日圆线虫诱导的肺部和空肠炎症期间，对脑-肠相互作用和肠道运动进行了研究。从感染前1天到感染后28天连续记录空肠肌电图活动。通过免疫组织化学评估大脑中c-fos的表达，并在感染后第1、7、14、21和28天测定肺和肠道中的髓过氧化物酶（MPO）活性。从第4天（对应幼虫迁移至肠道）到第14天，周期性肠道运动模式被不规则活动所取代。在第1天（巴西日圆线虫的肺部阶段），c-fos在孤束核（NTS）尾侧核和外侧臂旁核（LPB）中表达，在第7天在NTS内侧部分、LPB和蓝斑中表达。感染后第1天至21天，肺和肠道MPO活性增加。在巴西日圆线虫感染期间，c-fos表达表明在肺部和肠道炎症开始时特定且不同的脑核被激活，这与运动障碍有关。
