通过使用“科学96”转录图谱将ESTs定位到TSC1候选区间。

Mapping ESTs to the TSC1 candidate interval by use of the 'Science 96' transcript map.

作者信息

Wolfe J, Jeremiah S, Young J, Burley M W, Stewart H, McCulley M, Grant C, Naz K, Povey S

机构信息

Galton Laboratory, Department of Biology, University College London.

出版信息

Ann Hum Genet. 1997 Sep;61(Pt 5):401-9. doi: 10.1046/j.1469-1809.1997.6150401.x.

DOI:10.1046/j.1469-1809.1997.6150401.x

PMID:9459002

Abstract

The transcription map of the human genome published by Schuler et al. (1996) is a valuable resource in which approximately one quarter of all human genes have been mapped with respect to genetic framework markers using radiation hybrids. We have taken information from this map to provide potential genes within the TSC1 candidate region on chromosome 9q34. In so doing we have been able to provide an independent assay of the quality of the radiation hybrid mapping by using somatic cell hybrids and a 2 Mb cosmid contig covering the TSC1 region as mapping tools. In addition, we have built sequence contigs of ESTs for 25 clusters. This has shown that about 20% of the relevant EST clusters in the Unigene resource (Boguski & Schuler 1995) contain chimaeric clones.

摘要

舒勒等人（1996年）发表的人类基因组转录图谱是一份宝贵的资源，其中约四分之一的人类基因已通过辐射杂种相对于遗传框架标记进行了定位。我们从该图谱中获取信息，以提供9号染色体q34区域TSC1候选区域内的潜在基因。在此过程中，我们能够通过使用体细胞杂种和覆盖TSC1区域的2 Mb黏粒重叠群作为定位工具，对辐射杂种定位的质量进行独立分析。此外，我们构建了25个簇的EST序列重叠群。这表明，在Unigene资源（博古斯基和舒勒，1995年）中，约20%的相关EST簇包含嵌合克隆。