Hornigold N, van Slegtenhorst M, Nahmias J, Ekong R, Rousseaux S, Hermans C, Halley D, Povey S, Wolfe J
Galton Laboratory, Department of Biology, University College London, United Kingdom.
Genomics. 1997 May 1;41(3):385-9. doi: 10.1006/geno.1997.4681.
The disease gene TSC1 has been genetically mapped to human chromosome region 9q34, in a 4-cM interval between the markers D9S149 and D9S114. Within this interval there is conflicting genetic evidence as to the finer localization of the gene. We have used finger-printing methods and hybridization to produce a 1.7-Mb overlapping clone map covering the TSC1 candidate region, with a single gap of 20 kb. We have localized 12 previously cloned genes and 17 genetic markers on this map and have confirmed the order of the genetic map. This deep set of overlapping clones is now ready to be used for candidate gene isolation, for transcription studies, or for sequencing.
疾病基因TSC1已通过基因定位到人类染色体9q34区域，位于标记D9S149和D9S114之间4厘摩的区间内。在这个区间内，关于该基因更精细定位存在相互矛盾的遗传证据。我们利用指纹图谱法和杂交技术构建了一个覆盖TSC1候选区域的1.7兆碱基重叠克隆图谱，仅有一个20千碱基的缺口。我们已在该图谱上定位了12个先前克隆的基因和17个遗传标记，并确认了遗传图谱的顺序。这组深度重叠克隆现在可用于分离候选基因、进行转录研究或测序。
