Peters K G, Coogan A, Berry D, Marks J, Iglehart J D, Kontos C D, Rao P, Sankar S, Trogan E
Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.
Br J Cancer. 1998;77(1):51-6. doi: 10.1038/bjc.1998.8.
Endothelial receptor tyrosine kinases may play important roles in pathological vascular growth, particularly in tumours. In this study, immunohistochemistry was used to evaluate the expression of a novel endothelial receptor tyrosine kinase, Tie2/Tek, in the endothelium of vascular 'hotspots' in normal breast tissue (n = 10), benign breast lesions (n = 10) and in breast tumours (n = 123). Tie2 expression was detected in the endothelium of all breast tissues examined. However, the strongest expression of Tie-2 was seen in vascular 'hot spots' within the inflammatory infiltrate at the periphery of invasive tumours. Moreover, the proportion of Tie2-positive vessels (Tie2 counts/CD31 counts) was significantly higher in breast tumours than the proportion of Tie2-positive vessels in either normal breast tissue or benign breast lesions (P = 0.004 and 0.0001 respectively). These data are consistent with a role for Tie2 in tumour angiogenesis and demonstrate the potential use of Tie2 expression as a novel marker of the tumour vasculature.
内皮受体酪氨酸激酶可能在病理性血管生长中发挥重要作用，尤其是在肿瘤中。在本研究中，采用免疫组织化学方法评估一种新型内皮受体酪氨酸激酶Tie2/Tek在正常乳腺组织（n = 10）、乳腺良性病变（n = 10）和乳腺肿瘤（n = 123）血管“热点”内皮中的表达。在所检查的所有乳腺组织的内皮中均检测到Tie2表达。然而，在浸润性肿瘤周边炎症浸润内的血管“热点”中观察到Tie-2的最强表达。此外，乳腺肿瘤中Tie2阳性血管的比例（Tie2计数/CD31计数）显著高于正常乳腺组织或乳腺良性病变中Tie2阳性血管的比例（分别为P = 0.004和0.0001）。这些数据与Tie2在肿瘤血管生成中的作用一致，并证明Tie2表达作为肿瘤脉管系统新标志物的潜在用途。
