Bondarenko V M, Luchshev V I, Shakhmardanov M Z, Isaeva N P
Gamaleya Research Institute of Epidemiology and Microbiology, Russian Medical University, Moscow, Russia.
Zh Mikrobiol Epidemiol Immunobiol. 1997 Nov-Dec(6):81-3.
S. flexneri 2a outer membrane proteins of 38, 43, 62 and 78 kD, determined by the 140 mD invasiveness plasmid, serving as antigens and specific rabbit sera serving as antibodies were used for diagnosing S. flexneri infection in the enzyme immunoassay. The examination of 96 patients and 20 healthy donors showed the possibility of the detection of S. flexneri 2a protein invasins and antibodies to them at different periods of the disease. During the severe course of shigellosis a higher level of protein invasins in the blood serum of patients was established in comparison with that observed during the medium severe course. An elevated level of protein invasins in the blood serum was accompanied by a lower content of specific antibodies to them, which was probably due to the immunosuppressive action of the invasiveness plasmid, established in earlier experiments.
由140 MD侵袭性质粒决定的38、43、62和78 kD福氏志贺菌2a外膜蛋白作为抗原，特异性兔血清作为抗体，用于酶免疫测定中诊断福氏志贺菌感染。对96例患者和20名健康供者的检测表明，在疾病的不同阶段有可能检测到福氏志贺菌2a蛋白侵袭素及其抗体。与中度严重病程相比，在志贺菌病严重病程中患者血清中蛋白侵袭素水平更高。血清中蛋白侵袭素水平升高伴随着针对它们的特异性抗体含量降低，这可能是由于早期实验中确定的侵袭性质粒的免疫抑制作用。
