大蒜病毒X基因组的分子特征分析。

Molecular characterization of the garlic virus X genome.

作者信息

Song S I, Song J T, Kim C H, Lee J S, Choi Y D

机构信息

Department of Agricultural Chemistry, Seoul National University, Suwon, Korea.

出版信息

J Gen Virol. 1998 Jan;79 ( Pt 1):155-9. doi: 10.1099/0022-1317-79-1-155.

DOI:10.1099/0022-1317-79-1-155

PMID:9460937

Abstract

The complete nucleotide sequence of the cDNA genome for garlic virus X (GVX), one of the major viruses infecting garlic plants, was determined. GVX is a single-stranded positive-sense RNA virus consisting of 8106 nucleotides excluding the 3'-end poly(A) tail and contains six open reading frames (ORFs) which encode putative proteins of 174 kDa (ORF1), 26 kDa (ORF2), 12 kDa (ORF3), 32 kDa (ORF4), 26 kDa (ORF5) and 15 kDa (ORF6). The putative viral proteins show similarity to those of carlaviruses and potexviruses but show the highest homology to shallot virus X (ShVX). Even though the GVX genome contains most of the structural elements common to carlaviruses and potexviruses, it is distinguished from them by the presence of an ORF4 which encodes an unusual protein. These results suggest that GVX may belong to an unassigned group of ShVX and GarV-type viruses rather than to the carlaviruses or potexviruses.

摘要

确定了感染大蒜植株的主要病毒之一——大蒜病毒X（GVX）的cDNA基因组的完整核苷酸序列。GVX是一种单链正义RNA病毒，不包括3'端多聚（A）尾时由8106个核苷酸组成，含有六个开放阅读框（ORF），分别编码推定的174 kDa（ORF1）、26 kDa（ORF2）、12 kDa（ORF3）、32 kDa（ORF4）、26 kDa（ORF5）和15 kDa（ORF6）的蛋白质。推定的病毒蛋白与香石竹潜隐病毒属病毒和马铃薯X病毒属病毒的蛋白相似，但与青葱病毒X（ShVX）的同源性最高。尽管GVX基因组包含了香石竹潜隐病毒属病毒和马铃薯X病毒属病毒共有的大多数结构元件，但它因存在一个编码异常蛋白的ORF4而与它们不同。这些结果表明，GVX可能属于未分类的ShVX和GarV型病毒组，而不是香石竹潜隐病毒属病毒或马铃薯X病毒属病毒。