Song S I, Song J T, Chang M U, Lee J S, Choi Y D
Department of Agricultural Chemistry, Seoul National University, Suwon, Korea.
Mol Cells. 1997 Dec 31;7(6):705-9.
A partial cDNA clone for garlic virus X (GVX) was isolated. GVX was identified immunologically with an antibody raised against the recombinant coat protein (CP) and demonstrated to be one of the major viruses infecting garlic plants showing mosaic or streak symptoms. GVX belongs to an unassigned group of ShVX and GarV-type viruses rather than to carlaviruses or potexviruses. The recombinant CP of GVX was purified by Ni(2+)-NTA affinity chromatography. Anti-GVX CP antibody was raised against the purified recombinant CP. GVX particle is flexuous, rod-shaped, and about 750 nm long as determined by immunoelectron microscopy. The extent of infection by GVX of garlic plants was analyzed by Northern or immunoblot analyses of individual garlic plants cultivated in different regions. These results showed that almost all of the garlic plants tested from 40 different regions including America, China, Japan, and Korea are infected with GVX.
分离出了大蒜病毒X（GVX）的部分cDNA克隆。用针对重组外壳蛋白（CP）产生的抗体对GVX进行了免疫鉴定，结果表明它是感染出现花叶或条纹症状大蒜植株的主要病毒之一。GVX属于未分类的ShVX和GarV型病毒组，而非香石竹潜隐病毒属或马铃薯X病毒属。通过Ni(2+)-NTA亲和层析法纯化了GVX的重组CP。用纯化的重组CP制备了抗GVX CP抗体。通过免疫电子显微镜测定，GVX粒子呈弯曲的杆状，长约750 nm。通过对不同地区种植的单个大蒜植株进行Northern印迹或免疫印迹分析，分析了大蒜植株受GVX的感染程度。这些结果表明，来自包括美国、中国、日本和韩国在内的40个不同地区的几乎所有受试大蒜植株都感染了GVX。
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