Militão V, Moreno I, Rodríguez-Cerezo E, García-Arenal F
Departamento de Biotecnología, E.T.S. Ingenieros Agrónomos, Universidad Politécnica de Madrid, Spain.
J Gen Virol. 1998 Jan;79 ( Pt 1):177-84. doi: 10.1099/0022-1317-79-1-177.
The interactions of seven isolates of peanut stunt cucumovirus (PSV) originating from North America, Europe and Africa, and two variants of PSV satellite RNA (sat RNA) were analysed. Electrophoretic and immunoblot analyses of the coat protein (CP) and Northern blot hybridization analyses of the viral RNAs showed that isolates PSV F352, 1339 and 1507 belonged to subgroup I, and isolates PSV W, Su and B to subgroup II. The seventh isolate, robinia mosaic virus (RoMV) clustered with subgroup I isolates by CP analysis, but was related to both subgroups by RNA hybridization analysis. The ability to support the accumulation of two newly described sat RNA variants, P4 and P6 sat RNAs, was not related to PSV isolate classification: neither PSV W nor RoMV were helper viruses for these PSV sat RNAs. Symptom modulation by both sat RNAs was the same: the presence of sat RNA did not modify the symptoms induced by subgroup I isolates but exacerbated the symptoms induced by subgroup II isolates in both tobacco and cowpea. Sat RNAs P4 and P6 contained 393 nucleotides, and differed only in three nucleotide substitutions. This resulted in marked differences in infectivity, level of accumulation and relative encapsidation between both the sat RNAs. Accumulation levels and relative encapsidation of sat RNAs was also affected by the isolate of helper virus.
分析了来自北美、欧洲和非洲的7株花生矮化黄瓜花叶病毒（PSV）分离株以及两种PSV卫星RNA（sat RNA）变体之间的相互作用。对病毒外壳蛋白（CP）的电泳和免疫印迹分析以及对病毒RNA的Northern印迹杂交分析表明，PSV F352、1339和1507分离株属于I亚组，PSV W、Su和B分离株属于II亚组。通过CP分析，第七个分离株，刺槐花叶病毒（RoMV）与I亚组分离株聚类，但通过RNA杂交分析与两个亚组均相关。支持两种新描述的sat RNA变体P4和P6 sat RNA积累的能力与PSV分离株分类无关：PSV W和RoMV都不是这些PSV sat RNA的辅助病毒。两种sat RNA对症状的调节作用相同：sat RNA的存在不会改变I亚组分离株诱导的症状，但会加重II亚组分离株在烟草和豇豆中诱导产生的症状。P4和P6 sat RNA含有393个核苷酸，仅在三个核苷酸取代上有所不同。这导致两种sat RNA在感染性、积累水平和相对包装方面存在显著差异。sat RNA的积累水平和相对包装也受到辅助病毒分离株的影响。
