Japundzić M M, Bastomsky C H, Japundzić I P
Acta Endocrinol (Copenh). 1976 Jan;81(1):110-9. doi: 10.1530/acta.0.0810110.
Normal rats were treated with pregnenolone- 16alpha - carbonitrile (PCN) 10 mg/100 g by stomach tube twice daily for 3 days. In these animals the biliary excretion of intravenously injected 125I-thyroxine (T4) was enhanced and the bile: plasma 125I ratio (B/P ratio) and the biliary clearance rate of plasma 125I-T4 was increased. Normal rats were treated with PCN for 3 days and homozygous Gunn rats for 13 days. In both groups PCN enhanced the bile flow and elevated the B/P ratios and the biliary clearance rate of plasma T4 following ip injection of 125I-T4 17 h previously. PCN-treatment had no effect on the fractions of biliary 125I present as T4-glucuronide, T4 and I- in either the normal or Gunn rats. Treatment with PCN for 10 days produced goitres in normal and Gunn rats and in normal rats elevated the serum TSH (bioassay) levels and the 17 h thyroid 131I uptake as well as the serum PB125I concentrations, without affecting stable PBI concentrations. These data indicated increased pituitary TSH release in response to increased peripheral metabolism of thyroid hormone; enhanced hormonal release from the thyroid kept pace with the accelerated peripheral loss.
正常大鼠通过胃管给予孕烯醇酮-16α-腈（PCN），剂量为10mg/100g，每日两次，共3天。在这些动物中，静脉注射的125I-甲状腺素（T4）的胆汁排泄增强，胆汁与血浆的125I比率（B/P比率）以及血浆125I-T4的胆汁清除率增加。正常大鼠用PCN处理3天，纯合子Gunn大鼠用PCN处理13天。在两组中，PCN均增强了胆汁流量，并提高了17小时前腹腔注射125I-T4后的B/P比率以及血浆T4的胆汁清除率。PCN处理对正常或Gunn大鼠中以T4-葡萄糖醛酸苷、T4和I-形式存在的胆汁125I的比例没有影响。用PCN处理10天会使正常和Gunn大鼠出现甲状腺肿，并且在正常大鼠中会提高血清促甲状腺激素（生物测定）水平、17小时甲状腺131I摄取以及血清PB125I浓度，而不影响稳定的PBI浓度。这些数据表明，垂体促甲状腺激素释放增加是对甲状腺激素外周代谢增加的反应；甲状腺激素释放的增强与外周加速损失保持同步。
