从胚胎主动脉-性腺-中肾区域体外扩增小鼠多能造血祖细胞。

In vitro expansion of murine multipotential hematopoietic progenitors from the embryonic aorta-gonad-mesonephros region.

作者信息

Mukouyama Y, Hara T, Xu M, Tamura K, Donovan P J, Kim H, Kogo H, Tsuji K, Nakahata T, Miyajima A

机构信息

Institute of Molecular and Cellular Biosciences, The University of Tokyo, Japan.

出版信息

Immunity. 1998 Jan;8(1):105-14. doi: 10.1016/s1074-7613(00)80463-x.

DOI:10.1016/s1074-7613(00)80463-x

PMID:9462516

Abstract

The origin of hematopoietic stem cells (HSCs) and their growth factor requirement are poorly understood. Here we describe a new in vitro culture system of the aorta-gonad-mesonephros (AGM) region, where long-term repopulating HSCs first arise. We demonstrate that oncostatin M (OSM) is expressed in the AGM and is absolutely required for the expansion of multipotential hematopoietic progenitors in vitro. In addition, OSM enhances the formation of endothelial cell clusters. Thus, OSM appears to be a key cytokine for the development of multipotential hematopoietic progenitors in the AGM, possibly acting on common precursor cells between HSCs and endothelial cells. By using the AGM culture derived from macrophage colony-stimulating factor (M-CSF)-deficient op/op mutant embryos, we also show a pivotal role for M-CSF in fetal myelopoiesis.

摘要

造血干细胞（HSCs）的起源及其对生长因子的需求仍知之甚少。在此，我们描述了一种新的主动脉-性腺-中肾（AGM）区域体外培养系统，长期重建造血干细胞最初在此处产生。我们证明，抑瘤素M（OSM）在AGM中表达，并且是体外多能造血祖细胞扩增所绝对必需的。此外，OSM可增强内皮细胞簇的形成。因此，OSM似乎是AGM中多能造血祖细胞发育的关键细胞因子，可能作用于造血干细胞和内皮细胞之间的共同前体细胞。通过使用源自巨噬细胞集落刺激因子（M-CSF）缺陷型op/op突变胚胎的AGM培养物，我们还显示了M-CSF在胎儿髓系造血中的关键作用。