从多能干细胞生成HLF+HOXA+人类造血祖细胞的方案。
Protocol for the generation of HLF+ HOXA+ human hematopoietic progenitor cells from pluripotent stem cells.
作者信息
Zheng Sherry Li, Fowler Jonas L, Chen Julie Y, Li Christopher, Lin Elaine, Nguyen Alana T, Chen Angela, Daley George Q, Ang Lay Teng, Loh Kyle M
机构信息
Institute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Institute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
出版信息
STAR Protoc. 2025 Mar 21;6(1):103592. doi: 10.1016/j.xpro.2024.103592. Epub 2025 Jan 24.
Hematopoietic stem cells (HSCs) generate blood and immune cells. Here, we present a protocol to differentiate human pluripotent stem cells (hPSCs) into hematopoietic progenitors that express the signature HSC transcription factors HLF, HOXA5, HOXA7, HOXA9, and HOXA10. hPSCs are dissociated, seeded, and then sequentially differentiated into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and hematopoietic progenitors through the sequential addition of defined, serum-free media. This 10-day protocol enables the manufacturing of blood and immune cells in monolayer cultures. For complete details on the use and execution of this protocol, please refer to Fowler and Zheng et al..
造血干细胞（HSCs）可生成血液和免疫细胞。在此，我们展示了一种将人多能干细胞（hPSCs）分化为表达标志性造血干细胞转录因子HLF、HOXA5、HOXA7、HOXA9和HOXA10的造血祖细胞的方案。hPSCs先解离、接种，然后通过依次添加特定的无血清培养基，顺序分化为后原始条纹、侧中胚层、动脉内皮、造血内皮和造血祖细胞。这个为期10天的方案能够在单层培养中制造血液和免疫细胞。有关该方案使用和实施的完整详细信息，请参考福勒和郑等人的研究。
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