Zeng Z, Fan P, Rand E, Kyaw H, Su K, Madike V, Carter K C, Li Y
Human Genome Sciences Incorporated, Rockville, Maryland 20850, USA.
Biochem Biophys Res Commun. 1998 Jan 26;242(3):575-8. doi: 10.1006/bbrc.1997.7591.
With the use of the degenerated nucleotides that contain the conserved sequence of G protein-coupled receptor, we have identified a 648-bp clone (HDGRC02) from human genomic DNA with significant sequence homology to human neurotransmitter receptors. HDGRC02 was then used as a probe for the screening of full length gene. From human Lambda DASH II genomic library, a 1.6 Kb clone encoded a full length gene was isolated and named putative neurotransmitter receptor (PNR). PNR has a single open reading frame which predicts a 38.3 KD protein of 338 amino acids with seven transmembrane domain topography. The amino acid sequence of PNR exhibits considerable homology to the rat 5-HR1D receptor with 35% amino acid identity and 56% amino acid similarity. PNR also shows significant sequence homology to the 5-HT1D receptor from Japanese puffer fish fugu, to the 5-HT4L receptor from mouse, to the alpha-2 adrenergic receptor and to the D2 dopamine receptor. Northern blot analysis indicates that PNR is expressed in skeletal muscle and selected areas of the brain. A chromosome mapping study located the PNR gene with human chromosome band of 6q23. The findings in the present study demonstrate that PNR is a putative neurotransmitter receptor.
利用包含G蛋白偶联受体保守序列的简并核苷酸，我们从人类基因组DNA中鉴定出一个648碱基对的克隆（HDGRC02），它与人类神经递质受体具有显著的序列同源性。然后将HDGRC02用作筛选全长基因的探针。从人类λDASH II基因组文库中，分离出一个编码全长基因的1.6千碱基对克隆，并将其命名为假定神经递质受体（PNR）。PNR有一个单一的开放阅读框，预测编码一个由338个氨基酸组成的38.3千道尔顿的蛋白质，具有七个跨膜结构域拓扑结构。PNR的氨基酸序列与大鼠5-HR1D受体具有相当的同源性，氨基酸同一性为35%，氨基酸相似性为56%。PNR与日本河豚的5-HT1D受体、小鼠的5-HT4L受体、α-2肾上腺素能受体和D2多巴胺受体也显示出显著的序列同源性。Northern印迹分析表明PNR在骨骼肌和大脑的特定区域表达。一项染色体定位研究将PNR基因定位在人类6q23染色体带上。本研究的结果表明PNR是一种假定的神经递质受体。
