Tang B L, Low D Y, Lee S S, Tan A E, Hong W
Membrane Biology Laboratory, Institute of Molecular and Cell Biology, Singapore, Republic of Singapore.
Biochem Biophys Res Commun. 1998 Jan 26;242(3):673-9. doi: 10.1006/bbrc.1997.8029.
We have cloned a new member of the syntaxin family of proteins, designated human syntaxin 16 (hsyn16). The open reading frame encodes a polypeptide of 307 amino acids with potential coiled-coil domains and a carboxy-terminal hydrophobic tail, which is characteristic of other members of the syntaxin family. The encoded polypeptide bears sequence homology to known syntaxin molecules. Northern blot analysis revealed a single transcript that is fairly ubiquitous, being slightly more enriched in heart and pancreas. Indirect immunofluorescence localised myc-tagged hsyn16 (myc-hsyn16) to the Golgi apparatus, colocalizing well with lens culinaris agglutinin, an established Golgi marker, as well as with other Golgi SNAREs such as GS28 and syntaxin 5. Myc-hsyn16 is redistributed to the endoplasmic reticulum upon brefeldin A treatment, indicating that it is localised to the Golgi stack. The ubiquitous expression and Golgi localization of hsy16 suggest that it is involved in a vesicular transport step within the organelle.
我们克隆了 syntaxin 蛋白家族的一个新成员，命名为人类 syntaxin 16（hsyn16）。开放阅读框编码一个由 307 个氨基酸组成的多肽，该多肽具有潜在的卷曲螺旋结构域和一个羧基末端疏水尾，这是 syntaxin 家族其他成员的特征。编码的多肽与已知的 syntaxin 分子具有序列同源性。Northern 印迹分析显示有一个单一的转录本，它相当普遍存在，在心脏和胰腺中稍微富集一些。间接免疫荧光将 myc 标签的 hsyn16（myc-hsyn16）定位到高尔基体，与已确定的高尔基体标记物扁豆凝集素以及其他高尔基体 SNARE 蛋白如 GS28 和 syntaxin 5 共定位良好。用布雷菲德菌素 A 处理后，myc-hsyn16 重新分布到内质网，表明它定位于高尔基体堆叠。hsy16 的普遍表达和高尔基体定位表明它参与了该细胞器内的囊泡运输步骤。
