Jensen E C, Schrader H S, Rieland B, Thompson T L, Lee K W, Nickerson K W, Kokjohn T A
Department of Biology, College of Saint Benedict, Saint John's University, Collegeville, Minnesota 56321, USA.
Appl Environ Microbiol. 1998 Feb;64(2):575-80. doi: 10.1128/AEM.64.2.575-580.1998.
Two bacteriophage collections were examined with regard to their ability to form plaques on multiple bacterial host species. Nine of 10 phages studied were found to be broad-host-range bacteriophages. These phages fell into two groups. Group 1, the SN series, was isolated from sewage treatment plant samples with Sphaerotilus natans ATCC 13338 as a host. The DNAs of these bacteriophages contained modified bases and were insensitive to cleavage by type I and II restriction endonucleases. The efficiency of plating of these bacteriophages was changed only slightly on the alternate host. Group 2, the BHR series, was isolated by a two-host enrichment protocol. These bacteriophages were sensitive to restriction, and their efficiency of plating was dramatically reduced on the alternate host. Our results suggest that a multiple-host enrichment protocol may be more effective for the isolation of broad-host-range bacteriophages by avoiding the selection bias inherent in single-host methods. At least two of the broad-host-range bacteriophages mediated generalized transduction. We suggest that broad-host-range bacteriophages play a key role in phage ecology and gene transfer in nature.
对两个噬菌体文库进行了检测，以评估它们在多种细菌宿主物种上形成噬菌斑的能力。在所研究的10种噬菌体中，有9种被发现是广宿主范围噬菌体。这些噬菌体分为两组。第一组，即SN系列，是以浮游球衣菌ATCC 13338为宿主从污水处理厂样本中分离出来的。这些噬菌体的DNA含有修饰碱基，对I型和II型限制性内切酶的切割不敏感。这些噬菌体在交替宿主上的平板接种效率仅略有变化。第二组，即BHR系列，是通过双宿主富集方案分离出来的。这些噬菌体对限制敏感，它们在交替宿主上的平板接种效率显著降低。我们的结果表明，多宿主富集方案通过避免单宿主方法中固有的选择偏差，可能对广宿主范围噬菌体的分离更有效。至少有两种广宿主范围噬菌体介导了普遍性转导。我们认为广宿主范围噬菌体在自然界的噬菌体生态和基因转移中起关键作用。
