Ruhfel R E, Robillard N J, Thorne C B
J Bacteriol. 1984 Mar;157(3):708-11. doi: 10.1128/jb.157.3.708-711.1984.
Bacteriophage CP-51, a generalized transducing phage for Bacillus anthracis, B. cereus, and B. thuringiensis, mediates transduction of plasmid DNA. B. cereus GP7 harbors the 2.8-megadalton multicopy tetracycline resistance plasmid, pBC16. B. thuringiensis 4D11A carries pC194, the 1.8-megadalton multicopy chloramphenicol resistance plasmid. When phage CP-51 was propagated on these strains, it transferred the plasmid-encoded antibiotic resistances to the nonvirulent Weybridge (Sterne) strain of B. anthracis, to B. cereus 569, and to strains of several B. thuringiensis subspecies. The frequency of transfer was as high as 10(-5) transductants per PFU. Tetracycline-resistant and chloramphenicol-resistant transductants contained newly acquired plasmid DNA having the same molecular weight as that contained in the donor strain. Antibiotic-resistant transductants derived from any of the three species were effective donors of plasmids to recipients from all three species.
噬菌体CP - 51是一种针对炭疽芽孢杆菌、蜡样芽孢杆菌和苏云金芽孢杆菌的广义转导噬菌体，可介导质粒DNA的转导。蜡样芽孢杆菌GP7携带2.8兆道尔顿的多拷贝四环素抗性质粒pBC16。苏云金芽孢杆菌4D11A携带pC194，即1.8兆道尔顿的多拷贝氯霉素抗性质粒。当噬菌体CP - 51在这些菌株上繁殖时，它将质粒编码的抗生素抗性转移到炭疽芽孢杆菌的无毒韦布里奇（斯特恩）菌株、蜡样芽孢杆菌569以及几个苏云金芽孢杆菌亚种的菌株中。转移频率高达每噬菌斑形成单位10(-5)个转导子。四环素抗性和氯霉素抗性转导子含有新获得的质粒DNA，其分子量与供体菌株中所含的相同。源自这三个物种中任何一个的抗生素抗性转导子都是向所有三个物种的受体有效提供质粒的供体。
