Singh A, Singh S P
Human Genetics Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
Cancer Lett. 1998 Jan 9;122(1-2):151-6. doi: 10.1016/s0304-3835(97)00383-2.
The present study evaluates the potential of smokeless tobacco to translactationally modify the chemopreventive efficacy of phytic acid and butylated hydroxyanisole (BHA) via modulation of the hepatic xenobiotic detoxication system and antioxidant defense mechanism in the murine system. Phytic acid (1000 mg/kg b.w./day) by gavage while BHA (1% w/w) in diet induced a significant increase in the levels of glutathione-S-transferase (GST), acid soluble sulfhydryl (-SH), cytochrome b5 (Cyt. b5) and cytochrome P-450 (Cyt. P-450) in lactating dams and suckling pups. The hepatic levels of GST and -SH were significantly depressed whereas microsomal Cyt. b5, Cyt. P-450 and MDA levels were elevated in groups treated with smokeless tobacco (50 or 100 mg/kg b.w./day). The data reveals the inhibitory potential of smokeless tobacco on phytic acid-induced GST/GSH system efficiency besides the significant augmentation by smokeless tobacco on phytic acid or BHA-induced microsomal phase I enzymes. The direct or translactational modulation in the levels of xenobiotic detoxication system enzymes suggests the potential of smokeless tobacco to modify the chemopreventive efficacy of phytic acid or BHA.
本研究评估了无烟烟草通过调节小鼠系统中的肝脏外源性解毒系统和抗氧化防御机制，对植酸和丁基羟基茴香醚（BHA）的化学预防功效进行翻译后修饰的潜力。通过灌胃给予植酸（1000mg/kg体重/天），而在饮食中添加BHA（1%w/w）可使哺乳期母鼠和哺乳幼崽体内谷胱甘肽-S-转移酶（GST）、酸溶性巯基（-SH）、细胞色素b5（Cyt.b5）和细胞色素P-450（Cyt.P-450）的水平显著升高。在接受无烟烟草治疗的组中（50或100mg/kg体重/天），肝脏中GST和-SH的水平显著降低，而微粒体Cyt.b5、Cyt.P-450和丙二醛（MDA）的水平升高。数据显示，无烟烟草除了显著增强植酸或BHA诱导的微粒体I相酶外，还对植酸诱导的GST/GSH系统效率具有抑制作用。外源性解毒系统酶水平的直接或翻译后调节表明无烟烟草具有改变植酸或BHA化学预防功效的潜力。
