Mori K, Nakashima A, Nagatsu T, Ota A
Department of Physiology, School of Medicine, Fujita Health University, Toyoake, Aichi, Japan.
Neurosci Lett. 1997 Nov 28;238(1-2):21-4. doi: 10.1016/s0304-3940(97)00833-1.
The amounts of messenger RNA for three enzymes, namely guanosine triphosphate (GTP) cyclohydrolase 1,6-pyruvoyltetrahydropterin synthase, and sepiapterin reductase, all of which are involved in the de novo biosynthesis of (6R)-L-erythrodihydroxypropyl-2-amino-4-hydroxy-5,6,7,8-tetrahydro pteridine (BH4) from GTP, were measured quantitatively in murine neuroblastoma cell line N1E-115 by the competitive polymerase chain reaction (PCR) technique after reverse transcription using a heterologous DNA fragment as an internal standard. Twenty-four hour activation of this cell line with 1 microg/ml lipopolysaccharide resulted in statistically significant increases in the amounts of the messages of all three enzymes. Our data suggest that lipopolysaccharide can activate the intrinsic pathway resulting in the enhanced gene expression of these three enzymes in neuron-derived cells such as N1E-115.
通过竞争性聚合酶链反应（PCR）技术，以异源DNA片段作为内标，在逆转录后对鼠神经母细胞瘤细胞系N1E-115中三种酶的信使核糖核酸（mRNA）量进行了定量测定，这三种酶分别是鸟苷三磷酸（GTP）环化水解酶1、6-丙酮酸四氢蝶呤合酶和蝶呤还原酶，它们均参与从GTP从头生物合成（6R）-L-赤藓糖二羟基丙基-2-氨基-4-羟基-5,6,7,8-四氢蝶呤（BH4）的过程。用1微克/毫升脂多糖对该细胞系进行24小时激活后，所有这三种酶的信使量均出现具有统计学意义的增加。我们的数据表明，脂多糖可激活内在途径，导致在诸如N1E-115等神经元衍生细胞中这三种酶的基因表达增强。
