Mueller C G, Ho S, Massacrier C, Lebecque S, Liu Y J
Schering-Plough, Laboratory for Immunological Research, Dardilly, France.
Eur J Immunol. 1997 Dec;27(12):3130-4. doi: 10.1002/eji.1830271208.
A subtraction library of CD40-stimulated human tonsil dendritic cells has been constructed using a polymerase chain reaction approach adapted for low numbers of cells. From this library we identified a cDNA for a serine protease inhibitor, a serpin, which is absent from monocytes, B cells and T cells but expressed in CD40-activated monocyte- and progenitor cell-generated dendritic cells. In addition, the serpin is expressed in a lung fibroblast cell line and keratinocytes. Its mRNA is detected only in tonsil and thymus. The serpin described here reportedly functions as a megakaryocyte maturation factor in the presence of interleukin (IL)-3 and IL-11. This suggests that dendritic cells may promote the immune response by protecting IL-3 and IL-11 or other essential proteins from degradation.
利用适用于少量细胞的聚合酶链反应方法构建了CD40刺激的人扁桃体树突状细胞的消减文库。从该文库中，我们鉴定出一种丝氨酸蛋白酶抑制剂（一种丝氨酸蛋白酶抑制蛋白）的cDNA，单核细胞、B细胞和T细胞中不存在该cDNA，但在CD40激活的单核细胞和祖细胞生成的树突状细胞中表达。此外，该丝氨酸蛋白酶抑制蛋白在一种肺成纤维细胞系和角质形成细胞中表达。其mRNA仅在扁桃体和胸腺中检测到。据报道，这里描述的丝氨酸蛋白酶抑制蛋白在白细胞介素（IL）-3和IL-11存在的情况下作为巨核细胞成熟因子发挥作用。这表明树突状细胞可能通过保护IL-3和IL-11或其他必需蛋白不被降解来促进免疫反应。
