Dallongeville J, Baugé E, Lebel P, Fruchart J C
INSERM U-325, Département d'Athérosclérose, Institut Pasteur de Lille, France.
Eur J Clin Invest. 1997 Dec;27(12):1055-60. doi: 10.1046/j.1365-2362.1997.2350782.x.
Apolipoprotein (apo) C-III and apoE have a major influence on post-prandial apoB-containing lipoprotein metabolism. The goal of the present study was to compare the post-prandial changes in particles containing apoB and apoC-III and those containing apoB and apoE. Twenty subjects consumed a fatty meal (1 g of fat kg-1). Human lipoprotein particles were measured by enzyme-linked immunosorbent assay (ELISA) using combinations of anti-apoC-III, -apoE and -apoB. Post-prandial lipaemia was associated with an increase in LpC-III:B (+100%) and LpE:B (+55%; P < 0.05), which occurred 4.07 +/- 1.2 and 4.7 +/- 0.8 h after the meal respectively (P < 0.05). Gel filtration chromatography showed that fasting plasma LpC-III:B and LpE:B eluted in two fractions consisting of large and smaller sized particles; 3 h after the meal, LpC-III:B and LpE:B increased in the very low-density lipoprotein (VLDL) + intermediate-density lipoprotein (IDL) fraction; at 6 h, LpC-III:B and LpE:B decreased in VLDL and LpE:B increased moderately in the low-density lipoprotein (LDL) size range; at 10 h, both concentrations of lipoprotein particles returned to fasting levels. In conclusion, apoC-III-B-containing and apoE-B-containing lipoproteins have different post-prandial metabolic fates. These differences may result in different atherogenic potential.
载脂蛋白（apo）C-III和apoE对餐后含apoB的脂蛋白代谢有重大影响。本研究的目的是比较含apoB和apoC-III的颗粒以及含apoB和apoE的颗粒在餐后的变化。20名受试者食用了一顿高脂餐（1 g脂肪/kg体重）。使用抗apoC-III、-apoE和-apoB的组合，通过酶联免疫吸附测定（ELISA）法测量人脂蛋白颗粒。餐后血脂异常与LpC-III:B（升高100%）和LpE:B（升高55%；P<0.05）增加有关，分别在餐后4.07±1.2小时和4.7±0.8小时出现（P<0.05）。凝胶过滤色谱显示，空腹血浆LpC-III:B和LpE:B在由大小不同的颗粒组成的两个组分中洗脱；餐后3小时，LpC-III:B和LpE:B在极低密度脂蛋白（VLDL）+中间密度脂蛋白（IDL）组分中增加；6小时时，VLDL中的LpC-III:B和LpE:B降低，而在低密度脂蛋白（LDL）大小范围内LpE:B适度增加；10小时时，两种脂蛋白颗粒浓度均恢复到空腹水平。总之，含apoC-III-B和含apoE-B的脂蛋白有不同的餐后代谢命运。这些差异可能导致不同的致动脉粥样硬化潜力。
