Roth R I, Su D, Child A H, Wainwright N R, Levin J
Department of Pathology, University of California School of Medicine, Department of Veterans Affairs Medical Center, San Francisco 94121, USA.
J Infect Dis. 1998 Feb;177(2):388-94. doi: 10.1086/514204.
Limulus antilipopolysaccharide factor (LALF) can neutralize bacterial endotoxin, but its ability to prevent mortality following prolonged endotoxemia is unknown. Mice were challenged with an LD50 dose of intraperitoneal E. coli lipopolysaccharide (LPS) and then received LALF at various times after administration of LPS. Survival at 72 h was significantly improved by the administration of LALF at 4, 10, and even 24 h after LPS (73%, 78%, and 65% survival, respectively, vs. 15% survival in controls). Following intravenous administration of LALF at either 10 or 24 h after LPS, plasma levels of biologically active LPS abruptly fell (> 1000-fold lower than pre-LALF levels). Plasma LALF concentrations fell much more gradually in LPS-treated mice (t1/2 = 120 min) than in control mice (t1/2 = 2.5 min). In conclusion, LALF markedly decreased plasma concentrations of biologically active LPS and protected mice from lethality even when LALF was not administered until long after the onset of continuous endotoxemia.
鲎抗脂多糖因子（LALF）能够中和细菌内毒素，但其在长期内毒素血症后预防死亡的能力尚不清楚。给小鼠腹腔注射半数致死剂量的大肠杆菌脂多糖（LPS）进行攻击，然后在给予LPS后的不同时间给予LALF。在LPS注射后4小时、10小时甚至24小时给予LALF，72小时时的存活率显著提高（分别为73%、78%和65%的存活率，而对照组为15%的存活率）。在LPS注射后10小时或24小时静脉注射LALF后，具有生物活性的LPS血浆水平急剧下降（比注射LALF前的水平低1000倍以上）。LPS处理的小鼠血浆LALF浓度下降比对照小鼠（半衰期=2.5分钟）更缓慢（半衰期=120分钟）。总之，即使在持续内毒素血症发作很久之后才给予LALF，LALF也能显著降低具有生物活性的LPS的血浆浓度，并保护小鼠免于死亡。
