Verjans G M, Remeijer L, van Binnendijk R S, Cornelissen J G, Völker-Dieben H J, Baarsma S G, Osterhaus A D
Rotterdam Eye Hospital, Institute of Virology, Erasmus University Rotterdam, The Netherlands.
J Infect Dis. 1998 Feb;177(2):484-8. doi: 10.1086/517382.
Herpetic stromal keratitis (HSK) is a corneal disease initiated by a herpes simplex virus (HSV) infection with a postulated T cell-mediated immunopathology. To study the antigen specificity of cornea-infiltrating T cells in HSK patients, T cells were isolated and expanded by mitogenic stimulation from corneas of 2 patients with HSV-1-mediated HSK. A substantial number of the T cell clones (TCCs) obtained from these T cell lines were HSV-specific. All HSV-specific TCCs were of the CD3+CD4+CD8- phenotype. These TCCs responded to autologous HSV-infected corneal keratocytes, which expressed HLA class II molecules following incubation with interferon-gamma. Upon HSV-specific stimulation, all TCCs secreted interleukin-4, interleukin-5, and interferon-gamma. The data presented suggest that HSV-specific CD4+ T cells play a role in the immunopathogenesis of HSK in humans and that corneal keratocytes may act as antigen-presenting cells in this local T cell response.
疱疹性基质性角膜炎（HSK）是一种由单纯疱疹病毒（HSV）感染引发的角膜疾病，推测其具有T细胞介导的免疫病理机制。为研究HSK患者角膜浸润T细胞的抗原特异性，从2例由HSV-1介导的HSK患者的角膜中通过有丝分裂原刺激分离并扩增T细胞。从这些T细胞系获得的大量T细胞克隆（TCC）对HSV具有特异性。所有HSV特异性TCC均为CD3+CD4+CD8-表型。这些TCC对自体HSV感染的角膜角膜细胞有反应，后者在与γ干扰素孵育后表达HLA II类分子。在HSV特异性刺激下，所有TCC均分泌白细胞介素-4、白细胞介素-5和γ干扰素。所呈现的数据表明，HSV特异性CD4+T细胞在人类HSK的免疫发病机制中起作用，并且角膜角膜细胞可能在这种局部T细胞反应中充当抗原呈递细胞。
