Vispé S, Defais M
Institut de Pharmacologie et de Biologie Structurale, CNRS, UPR 9062, Toulouse, France.
Biochimie. 1997 Oct;79(9-10):587-92. doi: 10.1016/s0300-9084(97)82007-x.
During the last years, homologues of E coli RecA have been cloned in numerous species including man. These Rad51 proteins share sequence as well as functional homologies with the bacterial protein. Human Rad51 (HsRad51) is able to catalyze strand exchange in vitro between homologous DNAs, but with a lower efficiency compared to that of RecA. This suggests the requirement of additional factors. A very interesting feature of Rad51 is its essential role in mouse which could mean that it has gained an essential function in cell growth. The interaction of HsRad51 with several tumor suppressor genes namely p53, BRCA1 and BRCA2 implies possible role(s) of this protein in tumorigenesis. Thus, the continued study of Rad51 should bring important insights not only into homologous recombination mechanisms but also into cell proliferation regulation.
在过去几年中，大肠杆菌RecA的同源物已在包括人类在内的众多物种中被克隆出来。这些Rad51蛋白与细菌蛋白在序列和功能上具有同源性。人类Rad51（HsRad51）能够在体外催化同源DNA之间的链交换，但与RecA相比效率较低。这表明需要其他因子。Rad51一个非常有趣的特性是它在小鼠中具有重要作用，这可能意味着它在细胞生长中获得了一项重要功能。HsRad51与几个肿瘤抑制基因，即p53、BRCA1和BRCA2的相互作用暗示了该蛋白在肿瘤发生中可能发挥的作用。因此，对Rad51的持续研究不仅应能为同源重组机制带来重要见解，还能为细胞增殖调控带来重要见解。
