Takahashi K, Akiba Y, Tamura K
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, Japan.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1997 Nov;118(3):301-4. doi: 10.1016/s0742-8413(97)00166-7.
We determined whether dietary ascorbic acid (0.3 or 3 g/kg diet) modulates hepatic microsomal mixed function oxidase (MFO) system and plasma alpha 1 acid glycoprotein (AGP) concentration in chicks treated with Escherichia coli lipopolysaccharide (LPS). Injection of LPS (250 micrograms/kg body weight every other day) intraperitoneally for 14 days decreased cytochromes P450 and b, content and NADPH-cytochrome c reductase activity in hepatic microsomes in male broilers. Content of cytochromes P450 and b5 was negatively correlated with plasma AGP concentration. Feeding ascorbic acid partly alleviated the reduction of cytochromes P450 and b5 in males. Plasma AGP concentration also increased with the LPS injection and was partly lowered by feeding ascorbic acid. The results indicate that dietary ascorbic acid modulates the responses of the microsomal MFO system and of plasma AGP concentration against repeated injection of LPS in male broiler chicks.
我们研究了日粮中抗坏血酸（0.3或3克/千克日粮）是否会调节经大肠杆菌脂多糖（LPS）处理的雏鸡肝脏微粒体混合功能氧化酶（MFO）系统以及血浆α1酸性糖蛋白（AGP）浓度。每隔一天腹腔注射LPS（250微克/千克体重），持续14天，会降低雄性肉鸡肝脏微粒体中细胞色素P450和b5的含量以及NADPH-细胞色素c还原酶活性。细胞色素P450和b5的含量与血浆AGP浓度呈负相关。饲喂抗坏血酸可部分缓解雄性肉鸡中细胞色素P450和b5的减少。血浆AGP浓度也随着LPS注射而升高，而饲喂抗坏血酸可使其部分降低。结果表明，日粮中的抗坏血酸可调节雄性肉鸡雏鸡微粒体MFO系统以及血浆AGP浓度对反复注射LPS的反应。
