Takahashi K, Park J H, Akiba Y, Horiguchi M
Department of Animal Science, Faculty of Agriculture, Tohoku University, Sendai-shi, Japan.
Comp Biochem Physiol A Physiol. 1995 Jul;111(3):379-84. doi: 10.1016/0300-9629(95)00040-e.
Two experiments were undertaken to determine the effects of excess intake of protein and energy on hepatic microsomal mixed function oxidase (MFO) system in male broiler chickens. Two levels of protein (100 or 117% of ad libitum feeding) and energy (100 or 114% of ad libitum feeding) intake in factorial arrangements of treatments were used in experiment 1. Chicks (38 days of age) were provided the dietary treatments by tube-feeding for 8 days. In experiment 2, chicks were fed a control diet (23% protein and 3.2 Mcal metabolizable energy/kg diet) or a high protein and high energy (32% protein and 3.4 Mcal metabolizable energy/kg diet) ad libitum for 11 days. Energy intake over ad libitum feeding resulted in a reduction of cytochromes P-450 and b5 content. Excess intake of protein did not overcome the reduction of the cytochromes by overfeeding of energy. Feeding high protein diet with high energy content resulted in a reduction of the MFO system in the liver compared with feeding a control diet in the ad libitum condition. The results suggest that energy intake in broilers is the predominate modifier of hepatic microsomal MFO rather than protein intake under overfeeding conditions.
进行了两项实验，以确定雄性肉鸡过量摄入蛋白质和能量对肝脏微粒体混合功能氧化酶（MFO）系统的影响。实验1采用析因设计的两种蛋白质水平（随意采食的100%或117%）和能量水平（随意采食的100%或114%）。对38日龄雏鸡通过管饲法给予8天的日粮处理。在实验2中，雏鸡随意采食对照日粮（23%蛋白质和3.2兆卡代谢能/千克日粮）或高蛋白高能量日粮（32%蛋白质和3.4兆卡代谢能/千克日粮）11天。超过随意采食的能量摄入导致细胞色素P - 450和b5含量降低。蛋白质过量摄入并不能克服因能量过量饲喂导致的细胞色素减少。与随意采食对照日粮相比，采食高蛋白高能量日粮导致肝脏中MFO系统减少。结果表明，在过量饲喂条件下，肉鸡的能量摄入是肝脏微粒体MFO的主要调节因素，而非蛋白质摄入。
