Spectrum of cytokine gene expression in intestinal mucosal lesions of Crohn's disease and ulcerative colitis.
作者信息
Funakoshi K, Sugimura K, Anezaki K, Bannai H, Ishizuka K, Asakura H
机构信息
Third Department of Internal Medicine, Niigata University School of Medicine, Japan.
出版信息
Digestion. 1998;59(1):73-8. doi: 10.1159/000007470.
BACKGROUND/AIMS: We investigated the mRNA expression of spectrum of cytokines in the colonic mucosa in inflammatory bowel disease (IBD).
METHODS
The expression of cytokine gene was evaluated by using the reverse transcription-polymerase chain reaction and the radioactivity of amplified cDNA standardized by coamplified beta-actin cDNA.
RESULTS
Crohn's disease and ulcerative colitis showed significantly increased expression of IL-1beta, IL-6, IL-8 and TNF-alpha mRNA as compared with controls (p < 0.05).
CONCLUSION
Proinflammatory cytokines such as IL-1beta, IL-6, IL-8 and TNF-alpha are closely involved in the immune abnormalities of inflammatory mucosal lesions in IBD.
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