一种新的人肝外胆管癌细胞系（ICBD-1）的建立与鉴定

Establishment and characterization of a new human extrahepatic bile duct carcinoma cell line (ICBD-1).

作者信息

Takiyama I, Terashima M, Ikeda K, Kawamura H, Kashiwaba M, Tamura G, Suto T, Nakashima F, Sasaki R, Saito K

机构信息

Department of Surgery I, Iwate Medical University, School of Medicine, 19-1 Uchimaru, Marioka, 020, Japan.

出版信息

Oncol Rep. 1998 Mar-Apr;5(2):463-7. doi: 10.3892/or.5.2.463.

DOI:10.3892/or.5.2.463

PMID:9468580

Abstract

A new human extrahepatic bile duct carcinoma cell line (ICBD-1) was established from surgically resected tumor of a 71-year-old Japanese male patient. ICBD-1 cells proliferate in a layer with a population doubling time of 31.5 h and secrete tissue polypeptide antigen. ICBD-1 cells have a tetraploid pattern with a DNA index of 1.83 and chromosome counts showed equally distribution in a range from 65 to 69. IC50 values for ICBD-1 cells were 200 ng/ml for adriamycin, 400 ng/ml for mitomycin C, 2 microg/ml for cisplatin and 300 ng/ml for 5-fluorouracil. ICBD-1 cells were successfully transplanted to male nude mice, inducing progressive tumor growth. Histologically, nude mouse tumors were less differentiated than the original human tumor. Tumor cells showed alveolar structures with thin fibrous stroma, classified as poorly-differentiated adenocarcinoma. ICBD-1 is the fourth established cell line that originate from extrahepatic bile duct carcinoma and it will be applicable for the experimental studies of this disease.

摘要

一株新的人肝外胆管癌细胞系（ICBD - 1）从一名71岁日本男性患者手术切除的肿瘤中建立。ICBD - 1细胞呈单层增殖，群体倍增时间为31.5小时，并分泌组织多肽抗原。ICBD - 1细胞具有四倍体模式，DNA指数为1.83，染色体计数显示在65至69的范围内均匀分布。ICBD - 1细胞对阿霉素的IC50值为200 ng/ml，对丝裂霉素C为400 ng/ml，对顺铂为2 μg/ml，对5 - 氟尿嘧啶为300 ng/ml。ICBD - 1细胞成功移植到雄性裸鼠体内，导致肿瘤逐渐生长。组织学上，裸鼠肿瘤的分化程度低于原始人类肿瘤。肿瘤细胞呈现出具有薄纤维间质的腺泡结构，归类为低分化腺癌。ICBD - 1是第四株源自肝外胆管癌的建立细胞系，将适用于该疾病的实验研究。