Blanco M, Coello J, Iturriaga H, Maspoch S, Pérez-Maseda C
Departament de Química, Unitat de Química Analítica, Facultat de Ciències, Universitat Autònoma de Barcelona, Spain.
J Chromatogr A. 1998 Jan 9;793(1):165-75. doi: 10.1016/s0021-9673(97)00893-5.
A method for resolving the enantiomers of various 2-arylpropionic acids (viz. ketoprofen, ibuprofen and fenoprofen) by capillary zone electrophoresis (CZE) using a background electrolyte (BGE) containing a cyclodextrin as chiral selector is proposed. The effects of the type of cyclodextrin used and its concentration on resolution were studied and heptakis-2,3,6-tri- O-methyl-beta-cyclodextrin was found to be the sole effective choice for the quantitative enantiomeric resolution of all the compounds tested. The influence of pH, BGE concentration, capillary temperature and addition of methanol to the BGE on resolution and other separation-related parameters was also studied. The three compounds studied can be enantiomerically resolved with a high efficiency in a short time (less than 20 min) with no capillary treatment. This makes the proposed method suitable for assessing the enantiomeric purity of commercially available pharmaceuticals.
提出了一种通过毛细管区带电泳（CZE）使用含有环糊精作为手性选择剂的背景电解质（BGE）来拆分各种2-芳基丙酸（即酮洛芬、布洛芬和非诺洛芬）对映体的方法。研究了所用环糊精的类型及其浓度对拆分的影响，发现七（2,3,6-三-O-甲基）-β-环糊精是所有测试化合物定量对映体拆分的唯一有效选择。还研究了pH值、BGE浓度、毛细管温度以及向BGE中添加甲醇对拆分和其他与分离相关参数的影响。所研究的三种化合物无需对毛细管进行处理即可在短时间内（少于20分钟）高效地实现对映体拆分。这使得所提出的方法适用于评估市售药品的对映体纯度。
