Blanco M, Coello J, Iturriaga H, Maspoch S, Pérez-Maseda C
Departament de Química, Facultat de Ciències, Universitat Autònoma de Barcelona, Bellaterra, Spain.
J Chromatogr A. 1998 Mar 13;799(1-2):301-7. doi: 10.1016/s0021-9673(97)01040-6.
The new method for the enantiomeric resolution of various 2-arylpropionic acids by capillary zone electrophoresis (CZE) using heptakis-tri-O-methyl-beta-cyclodextrin as chiral selector was applied to the determination of ketoprofen in different commercially-available pharmaceutical preparations. The analyte was determined under chiral and nonchiral conditions (viz. in the presence and absence of 50 mM heptakis-tri-O-methyl-beta-cyclodextrin in the background electrolyte), with significantly similar results and relative standard deviations from 1.2 to 6.5% in both cases. The limits of detection and determination for the inactive enantiomer, R-(-)-ketoprofen, were calculated to be 7.0 x 10(-7) and 1.6 x 10(-6) M, respectively. The proposed method was successfully used to determine enantiomeric purity in the drugs studied, with results comparable to those provided by the chiral HPLC method.
采用七（三 - O - 甲基）-β-环糊精作为手性选择剂，通过毛细管区带电泳（CZE）对多种2 - 芳基丙酸进行对映体拆分的新方法，被应用于测定不同市售药物制剂中的酮洛芬。在有手性和非手性条件下（即在背景电解质中存在和不存在50 mM七（三 - O - 甲基）-β-环糊精的情况下）测定分析物，两种情况下结果显著相似，相对标准偏差为1.2%至6.5%。计算出非活性对映体R - (-)-酮洛芬的检测限和定量限分别为7.0×10⁻⁷ M和1.6×10⁻⁶ M。所提出的方法成功用于测定所研究药物的对映体纯度，结果与手性HPLC方法提供的结果相当。
