Jóźwik M, Jóźwik M, Jóźwik M, Szczypka M, Gajewska J, Laskowska-Klita T
Institute of Obstetrics and Gynaecology, School of Medicine, Białystok, Poland.
Clin Chim Acta. 1997 Nov 28;267(2):129-42. doi: 10.1016/s0009-8981(97)00148-4.
Blood collected routinely from donors, and preserved with CPDA-1 anticoagulant (citrate, phosphate, dextrose, adenine), was investigated. The concentration of reduced glutathione, and glutathione peroxidase, glutathione S-transferase, glutathione reductase, superoxide dismutase, and catalase activities in erythrocytes, as well as the total radical-trapping antioxidant parameter in plasma were determined on days 1, 3, 7, 12, 16, 20 and 25 of storage. At the end of the study, a 30% decrease in the reduced glutathione concentration (P < 0.001) and decreases in glutathione S-transferase (over 20%, P < 0.001), glutathione reductase (over 8%, P < 0.01) and superoxide dismutase (over 10%, P < 0.001) activities in erythrocytes, together with up to a 30% diminution of total antioxidant activity in plasma (P < 0.001) were noted. Thus, during blood storage, glutathione-dependent antioxidant systems in erythrocytes and antioxidant defence in plasma are depleted. From the present study, a twelve-day period can be considered a safe storage limit. The sequence of events occurring in stored blood, leading to peroxidative injury in erythrocytes, is discussed.
对常规采集自献血者并使用CPDA - 1抗凝剂（柠檬酸盐、磷酸盐、葡萄糖、腺嘌呤）保存的血液进行了研究。在储存的第1、3、7、12、16、20和25天，测定了红细胞中还原型谷胱甘肽的浓度以及谷胱甘肽过氧化物酶、谷胱甘肽S - 转移酶、谷胱甘肽还原酶、超氧化物歧化酶和过氧化氢酶的活性，以及血浆中的总自由基捕获抗氧化参数。在研究结束时，发现红细胞中还原型谷胱甘肽浓度下降了30%（P < 0.001），谷胱甘肽S - 转移酶（超过20%，P < 0.001）、谷胱甘肽还原酶（超过8%，P < 0.01）和超氧化物歧化酶（超过10%，P < 0.001）的活性下降，同时血浆中的总抗氧化活性降低了30%（P < 0.001）。因此，在血液储存期间，红细胞中依赖谷胱甘肽的抗氧化系统和血浆中的抗氧化防御功能会被耗尽。从本研究来看，12天的时间段可被视为安全储存期限。本文讨论了储存血液中发生的一系列导致红细胞过氧化损伤的事件。
