Kobayashi K, Muramori F, Aoki T, Hayashi M, Miyazu K, Fukutani Y, Mukai M, Koshino F
Department of Neuropsychiatry, Kanazawa University School of Medicine, Japan.
Dement Geriatr Cogn Disord. 1998 Jan-Feb;9(1):13-9. doi: 10.1159/000017015.
KP-1 immunostaining with microwave pretreatment in formalin-fixed, paraffin-embedded sections enhanced its immunoreactivity revealing extraneuronal neurofibrillary tangles (NFTs) called ghost tangles, senile plaques (SPs) and perivascular deposits as well as microglial labelling in Alzheimer-diseased brains. KP-1 stained cored and uncored SPs, granules within the SPs, perivascular beta-amyloid protein (beta AP) and star-like beta AP deposits in cortical layer I, which was confirmed in comparison to silver-impregnated structures in the Reusche-stained or Gallyas-Schiff-stained sections. On double immunostaining with KP-1 and ubiquitin, ghost tangles were labelled by KP-1 and intraneuronal NFTs were positive for ubiquitin. A few KP-1-positive granules deposits different from amyloid core were found within the SPs and the outer margin of amyloid cores of SPs were stained by KP-1. KP-1-positive microglia were attached to the ubiquitin-positive intraneuronal NFTs. Microglia were more numerously labelled by CR3/43 than by KP-1, and CR3/43-positive microglia were found to be preferentially attached to SPs. As KP-1 recognizes lysosome-associated antigen CD68, similarities between KP-1 positivity and Reusche-stained structures suggested that lysosomal activity was associated with beta AP deposits and ghost tangles were involved in lysosome-associated processes. It is speculated that lysosomes play a role in the process of ghost tangle formation and in beta AP deposits leading to SP formation.
在福尔马林固定、石蜡包埋切片中，采用微波预处理的KP-1免疫染色增强了其免疫反应性，揭示了阿尔茨海默病大脑中的细胞外神经原纤维缠结（NFTs），即所谓的幽灵缠结、老年斑（SPs）和血管周围沉积物以及小胶质细胞标记。KP-1对有核心和无核心的SPs、SPs内的颗粒、血管周围β-淀粉样蛋白（β-AP）以及皮质I层中的星状β-AP沉积物进行染色，与Reusche染色或Gallyas-Schiff染色切片中的银浸染结构相比得到了证实。用KP-1和泛素进行双重免疫染色时，幽灵缠结被KP-1标记，而神经元内NFTs对泛素呈阳性。在SPs内发现了一些与淀粉样核心不同的KP-1阳性颗粒沉积物，并且SPs淀粉样核心的外边缘被KP-1染色。KP-1阳性小胶质细胞附着于泛素阳性的神经元内NFTs。与KP-1相比，CR3/43标记的小胶质细胞数量更多，并且发现CR3/43阳性小胶质细胞优先附着于SPs。由于KP-1识别溶酶体相关抗原CD68，KP-1阳性与Reusche染色结构之间的相似性表明溶酶体活性与β-AP沉积物相关，并且幽灵缠结参与了溶酶体相关过程。据推测，溶酶体在幽灵缠结形成过程以及导致SP形成的β-AP沉积物过程中发挥作用。
