French K A, Murphy J A, Szczupak L
Department of Biology, University of California, San Diego, La Jolla 92093-0357, USA.
J Neurobiol. 1998 Jan;34(1):55-68.
The development of many neurons, including the Retzius (Rz) neurons of the medicinal leech, is shaped in part by interactions with other cells in the environment. To explore the nature of the interaction between growing Rz processes and potential target tissues, adult Rz neurons were cultured directly in contact with some of the tissues that normally serve as their targets in vivo. The morphology of the regenerated processes of these neurons varied depending upon the identity of the target tissues, but other cellular properties remained unchanged. In particular, although during normal development contact with peripheral targets determines the sign of Rz neurons' response to acetylcholine (ACh) applied to the soma, these cultured neurons maintained their original response to ACh even after as long as 2 weeks in culture on novel targets. Hence, some features of cultured adult Rz neurons varied depending upon the conditions, whereas other features remained fixed.
许多神经元的发育，包括药用水蛭的雷丘斯（Rz）神经元，部分是由与环境中其他细胞的相互作用所塑造的。为了探究生长中的Rz突起与潜在靶组织之间相互作用的本质，将成年Rz神经元直接与一些在体内通常作为其靶标的组织一起培养。这些神经元再生突起的形态因靶组织的不同而有所变化，但其他细胞特性保持不变。特别是，尽管在正常发育过程中与外周靶标的接触决定了Rz神经元对施加于胞体的乙酰胆碱（ACh）的反应信号，但这些培养的神经元即使在新靶标上培养长达2周后，仍保持其对ACh的原始反应。因此，培养的成年Rz神经元的一些特征因条件而异，而其他特征则保持不变。
