Touze A, Coursaget P
Institut de Virologie de Tours and CJF INSERM d'Immunologie des Maladies Infectieuses, Faculté des Sciences Pharmaceutiques 'Philippe Maupas', 31 avenue Monge, 37200 Tours, France.
Nucleic Acids Res. 1998 Mar 1;26(5):1317-23. doi: 10.1093/nar/26.5.1317.
Recombinant papillomavirus-like particles have recently been shown to be highly effective for the prevention of papillomavirus infections and associated tumors, and a virus-like particle-based vaccine against the most prevalent HPV causing genital infection in humans will be developed in the near future. Another use of these virus-like particles may lie in gene therapy and DNA immunization. We report here that human papillomavirus-like particles composed of the major capsid protein (L1) of HPV-16 are able to package unrelated plasmid DNA in vitro and then to deliver this foreign DNA to eukaryotic cells with the subsequent expression of the encoded gene. The results indicate higher gene transfer than with DNA alone or with liposome. Virus-like particles are a very promising vehicle for delivering genetic material into target cells. Moreover, the preparation of the gene transfer vehicle is relatively easy.
重组乳头瘤病毒样颗粒最近已被证明对预防乳头瘤病毒感染及相关肿瘤非常有效，并且在不久的将来将开发一种基于病毒样颗粒的疫苗，用于预防人类生殖器感染中最常见的人乳头瘤病毒（HPV）。这些病毒样颗粒的另一个用途可能在于基因治疗和DNA免疫。我们在此报告，由HPV-16主要衣壳蛋白（L1）组成的人乳头瘤病毒样颗粒能够在体外包装无关的质粒DNA，然后将这种外源DNA递送至真核细胞，并随后表达编码的基因。结果表明，与单独使用DNA或脂质体相比，基因转移效率更高。病毒样颗粒是将遗传物质递送至靶细胞的非常有前景的载体。此外，基因转移载体的制备相对容易。
