病毒样颗粒在生物学和医学中的不同应用：疫苗接种和递送系统。
Different applications of virus-like particles in biology and medicine: Vaccination and delivery systems.
作者信息
Shirbaghaee Zeinab, Bolhassani Azam
机构信息
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Biopolymers. 2016 Mar;105(3):113-32. doi: 10.1002/bip.22759.
Abstract
Virus-like particles (VLPs) mimic the whole construct of virus particles devoid of viral genome as used in subunit vaccine design. VLPs can elicit efficient protective immunity as direct immunogens compared to soluble antigens co-administered with adjuvants in several booster injections. Up to now, several prokaryotic and eukaryotic systems such as insect, yeast, plant, and E. coli were used to express recombinant proteins, especially for VLP production. Recent studies are also generating VLPs in plants using different transient expression vectors for edible vaccines. VLPs and viral particles have been applied for different functions such as gene therapy, vaccination, nanotechnology, and diagnostics. Herein, we describe VLP production in different systems as well as its applications in biology and medicine.
摘要
病毒样颗粒（VLPs）模仿病毒颗粒的整体结构，但不含病毒基因组，用于亚单位疫苗设计。与在多次加强注射中与佐剂共同施用的可溶性抗原相比，VLPs作为直接免疫原可引发高效的保护性免疫。到目前为止，几种原核和真核系统，如昆虫、酵母、植物和大肠杆菌，被用于表达重组蛋白，特别是用于生产VLPs。最近的研究也在利用不同的瞬时表达载体在植物中生产VLPs用于可食用疫苗。VLPs和病毒颗粒已被应用于不同的功能，如基因治疗、疫苗接种、纳米技术和诊断。在此，我们描述了VLPs在不同系统中的生产及其在生物学和医学中的应用。
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