Simpson H, Berthemy A, Buhrman D, Burton R, Newton J, Kealy M, Wells D, Wu D
Sanofi Pharmaceuticals, Inc., Malvern, PA 19355, USA.
Rapid Commun Mass Spectrom. 1998;12(2):75-82. doi: 10.1002/(SICI)1097-0231(19980131)12:2<75::AID-RCM112>3.0.CO;2-C.
The 96 well solid phase extraction (SPE) operated by robot in the LC/MS/MS bioanalysis offered rapid sample preparation for drugs and metabolites in biological matrices, based on simultaneous extraction of 96 samples. The use of a disk as sorbent in the 96 well plate further improved the performance of SPE and allowed for small elution volumes, making it possible to 'dilute and shoot" after SPE elution. In this study, a 96 well plate (Empore) was developed, characterized and optimized for several pharmaceutical compounds. In addition, a robot (MultiProbe) was modified to automate the 96 well plate operation. Examples were given to illustrate the major differences of using 96 well disk plate SPE in the method development as compared to the traditional SPE. This technology has been successfully used to support many clinical studies. Typically, a batch of 96 samples were prepared in 1-1.5 hours unattended (except for the replacement of a collection plate). Considerable savings in disposable supplies were also noted.
液相色谱串联质谱生物分析中由机器人操作的96孔固相萃取（SPE），基于对96个样品的同时萃取，为生物基质中的药物和代谢物提供了快速的样品制备。在96孔板中使用盘状吸附剂进一步提高了固相萃取的性能，并允许使用小体积洗脱液，使得在固相萃取洗脱后能够“稀释后进样”。在本研究中，开发了一种针对几种药物化合物的96孔板（Empore），对其进行了表征和优化。此外，对一台机器人（MultiProbe）进行了改装，以实现96孔板操作的自动化。给出了实例，以说明与传统固相萃取相比，在方法开发中使用96孔盘状板固相萃取的主要差异。该技术已成功用于支持多项临床研究。通常，一批96个样品在无人值守的情况下1 - 1.5小时内即可制备完成（更换收集板除外）。还注意到一次性耗材有可观的节省。
