• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Microsome biocolloids for rapid drug metabolism and inhibition assessment by LC-MS.用于通过液相色谱-质谱联用快速评估药物代谢和抑制作用的微粒体生物胶体
Drug Metab Lett. 2008 Aug;2(3):158-62. doi: 10.2174/187231208785425854.
2
Distinct effects of form selective cytochrome P450 inhibitors on cytochrome P450-mediated monooxygenase and hydrogen peroxide generating NADPH oxidase.选择性细胞色素 P450 抑制剂对细胞色素 P450 介导的单加氧酶和产生过氧化氢的 NADPH 氧化酶的不同影响。
Toxicol Appl Pharmacol. 2022 Nov 15;455:116258. doi: 10.1016/j.taap.2022.116258. Epub 2022 Sep 26.
3
[Metabolites and metabolic pathways of mesaconitine in rat liver microsomal investigated by using UPLC-MS/MS method in vitro].[采用超高效液相色谱-串联质谱法体外研究大鼠肝微粒体中中乌头碱的代谢产物及代谢途径]
Yao Xue Xue Bao. 2013 Dec;48(12):1823-8.
4
Polysaccharide peptides from Coriolus versicolor competitively inhibit model cytochrome P450 enzyme probe substrates metabolism in human liver microsomes.云芝多糖肽竞争性抑制人肝微粒体中模型细胞色素 P450 酶探针底物的代谢。
Phytomedicine. 2012 Mar 15;19(5):457-63. doi: 10.1016/j.phymed.2011.09.077. Epub 2012 Feb 2.
5
Cytochrome P450 2E1 is the primary enzyme responsible for low-dose carbon tetrachloride metabolism in human liver microsomes.细胞色素P450 2E1是负责人类肝微粒体中低剂量四氯化碳代谢的主要酶。
Chem Biol Interact. 2000 Mar 15;125(3):233-43. doi: 10.1016/s0009-2797(00)00149-6.
6
Bioactivation to free radicals and cytotoxicity of 1,1-dichloro-1-fluoroethane (HCFC-141b).1,1-二氯-1-氟乙烷（HCFC-141b）的自由基生物活化作用及细胞毒性
Xenobiotica. 2001 Feb;31(2):99-112. doi: 10.1080/00498250010031656.
7
Revisiting the metabolism of ketoconazole using accurate mass.
Drug Metab Lett. 2009 Aug;3(3):191-8. doi: 10.2174/187231209789352085.
8
Development and full validation of six inhibition assays for five major cytochrome P450 enzymes in human liver microsomes using an automated 96-well microplate incubation format and LC-MS/MS analysis.使用自动96孔微孔板孵育形式和液相色谱-串联质谱分析，开发并全面验证人肝微粒体中五种主要细胞色素P450酶的六种抑制测定法。
J Pharm Biomed Anal. 2007 May 9;44(1):211-23. doi: 10.1016/j.jpba.2007.02.034. Epub 2007 Mar 3.
9
Rapid LC-MS drug metabolite profiling using microsomal enzyme bioreactors in a parallel processing format.采用平行处理格式的微粒体酶生物反应器进行快速 LC-MS 药物代谢产物分析。
Anal Chem. 2009 Dec 15;81(24):9921-9. doi: 10.1021/ac9015853.
10
In vitro inhibitory effects of dihydromyricetin on human liver cytochrome P450 enzymes.二氢杨梅素对人肝脏细胞色素P450酶的体外抑制作用。
Pharm Biol. 2017 Dec;55(1):1868-1874. doi: 10.1080/13880209.2017.1339284.

引用本文的文献

1
Organ-Specific Screening for Protein Damage Using Magnetic Bead Bioreactors and LC-MS/MS.使用磁珠生物反应器和 LC-MS/MS 进行器官特异性蛋白质损伤筛选
Anal Chem. 2020 Apr 7;92(7):5337-5345. doi: 10.1021/acs.analchem.9b05871. Epub 2020 Mar 24.
2
Microfluidic array for simultaneous detection of DNA oxidation and DNA-adduct damage.微流控芯片阵列用于同时检测 DNA 氧化和 DNA 加合物损伤。
Analyst. 2016 Oct 21;141(20):5722-5729. doi: 10.1039/c6an01237j. Epub 2016 Aug 12.
3
State-of-the-Art Metabolic Toxicity Screening and Pathway Evaluation.最新代谢毒性筛选及途径评估。
Anal Chem. 2016 May 3;88(9):4584-99. doi: 10.1021/acs.analchem.5b04772. Epub 2016 Apr 14.
4
Elucidating Organ-Specific Metabolic Toxicity Chemistry from Electrochemiluminescent Enzyme/DNA Arrays and Bioreactor Bead-LC-MS/MS.通过电化学发光酶/DNA阵列和生物反应器珠粒液相色谱-串联质谱法阐明器官特异性代谢毒性化学
Chem Sci. 2015;6(4):2457-2468. doi: 10.1039/C4SC03401E.
5
Thin multicomponent films for functional enzyme devices and bioreactor particles.用于功能性酶装置和生物反应器颗粒的超薄多组分薄膜。
Soft Matter. 2014 Nov 7;10(41):8145-56. doi: 10.1039/c4sm01679c.
6
High-throughput metabolic genotoxicity screening with a fluidic microwell chip and electrochemiluminescence.高通量代谢遗传毒性筛选与流体微井芯片和电化学发光。
Lab Chip. 2013 Dec 7;13(23):4554-62. doi: 10.1039/c3lc50698c.
7
Comparison of DNA-Reactive Metabolites from Nitrosamine and Styrene Using Voltammetric DNA/Microsomes Sensors.使用伏安法DNA/微粒体传感器比较亚硝胺和苯乙烯的DNA反应性代谢物
Electroanalysis. 2009 May 1;21(9):1005-1013. doi: 10.1002/elan.200804521. Epub 2009 Mar 12.
8
Metabolic toxicity screening using electrochemiluminescence arrays coupled with enzyme-DNA biocolloid reactors and liquid chromatography-mass spectrometry.基于电化学发光阵列与酶-DNA 生物胶粒反应器和液相色谱-质谱联用的代谢毒性筛选
Annu Rev Anal Chem (Palo Alto Calif). 2012;5(1):79-105. doi: 10.1146/annurev.anchem.111808.073659. Epub 2012 Apr 5.
9
Microfluidic electrochemical array for detection of reactive metabolites formed by cytochrome P450 enzymes.用于检测细胞色素 P450 酶形成的反应代谢物的微流控电化学阵列。
Anal Chem. 2011 Dec 15;83(24):9499-506. doi: 10.1021/ac202269t. Epub 2011 Nov 15.
10
Efficient bioelectronic actuation of the natural catalytic pathway of human metabolic cytochrome P450s.高效生物电子学激活人体代谢细胞色素 P450s 的天然催化途径。
J Am Chem Soc. 2011 Feb 9;133(5):1459-65. doi: 10.1021/ja108637s. Epub 2011 Jan 7.

本文引用的文献

1
Enzyme-DNA biocolloids for DNA adduct and reactive metabolite detection by chromatography-mass spectrometry.用于通过色谱-质谱法检测DNA加合物和活性代谢物的酶-DNA生物胶体
Anal Chem. 2008 Feb 15;80(4):922-32. doi: 10.1021/ac702025f. Epub 2008 Jan 25.
2
Genotoxicity screening for N-nitroso compounds. Electrochemical and electrochemiluminescent detection of human enzyme-generated DNA damage from N-nitrosopyrrolidine.N-亚硝基化合物的遗传毒性筛选。N-亚硝基吡咯烷对人酶促DNA损伤的电化学和电化学发光检测。
Chem Commun (Camb). 2007 May 7(17):1713-5. doi: 10.1039/b703012f. Epub 2007 Apr 3.
3
Toxicity screening using biosensors that measure DNA damage.使用测量DNA损伤的生物传感器进行毒性筛选。
Curr Opin Drug Discov Devel. 2007 Jan;10(1):67-73.
4
Electrochemiluminescent arrays for cytochrome P450-activated genotoxicity screening. DNA damage from benzo[a]pyrene metabolites.用于细胞色素P450激活的遗传毒性筛选的电化学发光阵列。苯并[a]芘代谢物引起的DNA损伤。
Anal Chem. 2007 Mar 1;79(5):1897-906. doi: 10.1021/ac061975q. Epub 2007 Jan 30.
5
Cytochrome P450 (CYP) inhibition screening: comparison of three tests.细胞色素P450（CYP）抑制筛选：三种测试方法的比较。
Eur J Pharm Sci. 2006 Oct 1;29(2):130-8. doi: 10.1016/j.ejps.2006.06.005. Epub 2006 Jun 27.
6
Cytochrome P450s and other enzymes in drug metabolism and toxicity.细胞色素P450及其他参与药物代谢和毒性的酶。
AAPS J. 2006 Mar 10;8(1):E101-11. doi: 10.1208/aapsj080112.
7
In silico and in vitro screening for inhibition of cytochrome P450 CYP3A4 by comedications commonly used by patients with cancer.通过癌症患者常用的合并用药进行细胞色素P450 CYP3A4抑制的计算机模拟和体外筛选。
Drug Metab Dispos. 2006 Apr;34(4):534-8. doi: 10.1124/dmd.105.007625. Epub 2006 Jan 13.
8
Genotoxicity screening using biocatalyst/DNA films and capillary LC-MS/MS.使用生物催化剂/DNA膜和毛细管液相色谱-串联质谱法进行遗传毒性筛选。
Anal Chem. 2006 Jan 15;78(2):624-7. doi: 10.1021/ac0517996.
9
Role of metabolism in drug-induced idiosyncratic hepatotoxicity.代谢在药物性特异质性肝毒性中的作用。
Crit Rev Toxicol. 2005 Apr-May;35(4):325-61. doi: 10.1080/10408440590935620.
10
Elucidating mechanisms of drug-induced toxicity.阐明药物诱导毒性的机制。
Nat Rev Drug Discov. 2005 May;4(5):410-20. doi: 10.1038/nrd1720.

用于通过液相色谱-质谱联用快速评估药物代谢和抑制作用的微粒体生物胶体

Microsome biocolloids for rapid drug metabolism and inhibition assessment by LC-MS.

作者信息

Bajrami Besnik, Krishnan Sadagopan, Rusling James F

机构信息

Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060. USA.

出版信息

Drug Metab Lett. 2008 Aug;2(3):158-62. doi: 10.2174/187231208785425854.

DOI:10.2174/187231208785425854
PMID:19356087
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3473178/
Abstract

Rat liver microsomes attached to nanoparticles were used for LC-MS studies of CYP3A and 2E1 enzymes in metabolism of N-nitroso compounds. Using these biocolloids, turnover rates were measured within 2 min. Inhibitor IC(50) values for ketoconazole (KET) and 4-methylpyrazole (4-MEP) were estimated.

摘要

附着于纳米颗粒的大鼠肝微粒体用于N-亚硝基化合物代谢中CYP3A和2E1酶的液相色谱-质谱联用（LC-MS）研究。使用这些生物胶体，在2分钟内测量了周转率。估算了酮康唑（KET）和4-甲基吡唑（4-MEP）的抑制剂半数抑制浓度（IC50）值。