用于通过液相色谱-质谱联用快速评估药物代谢和抑制作用的微粒体生物胶体
Microsome biocolloids for rapid drug metabolism and inhibition assessment by LC-MS.
作者信息
Bajrami Besnik, Krishnan Sadagopan, Rusling James F
机构信息
Department of Chemistry, University of Connecticut, Storrs, Connecticut 06269-3060. USA.
出版信息
Drug Metab Lett. 2008 Aug;2(3):158-62. doi: 10.2174/187231208785425854.
Abstract
Rat liver microsomes attached to nanoparticles were used for LC-MS studies of CYP3A and 2E1 enzymes in metabolism of N-nitroso compounds. Using these biocolloids, turnover rates were measured within 2 min. Inhibitor IC(50) values for ketoconazole (KET) and 4-methylpyrazole (4-MEP) were estimated.
摘要
附着于纳米颗粒的大鼠肝微粒体用于N-亚硝基化合物代谢中CYP3A和2E1酶的液相色谱-质谱联用（LC-MS）研究。使用这些生物胶体，在2分钟内测量了周转率。估算了酮康唑（KET）和4-甲基吡唑（4-MEP）的抑制剂半数抑制浓度（IC50）值。
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