紫外线和微波消毒技术用于牙刷去污的比较评估

Comparative evaluation of ultraviolet and microwave sanitization techniques for toothbrush decontamination.

作者信息

Gujjari S K, Gujjari A K, Patel P V, Shubhashini P V

机构信息

Department of Periodontology, JSS Dental College & Hospital, Karnataka, Constituent College of JSS University, Mysore, India.

Department of Prosthodontics, JSS Dental College & Hospital, Karnataka, Constituent College of JSS University, Mysore, India.

出版信息

J Int Soc Prev Community Dent. 2011 Jan;1(1):20-6. doi: 10.4103/2231-0762.86383.

DOI:10.4103/2231-0762.86383

PMID:24478949

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3894078/

Abstract

BACKGROUND

Toothbrushes are rapidly contaminated with different microorganisms representing a possible cause of infection or reinfection especially in the periodontal patients under therapy. The purpose of this study was to evaluate the sanitization of toothbrushes previously contaminated by various oral microorganisms using a domestic microwave oven and commercial ultraviolet (UV) light toothbrush sanitizer.

MATERIALS AND METHODS

Thirty male dental graduates were randomly assigned to control or experimental groups and received standardized toothbrushes for home use. Each subject was instructed to use it with the standardized modified Bass technique for 1 week and submit it to the investigator after use. Collected toothbrushes were cultured and analyzed for the number of colony-forming units (CFUs). In the next phase, once again a new set of toothbrush was given to each subject and instructed to use it for one more week and follow the same instructions as given earlier. Subsequently, the used toothbrushes were again collected and were sanitized by microwave irradiation, UV radiation, or were not sanitized (control group). After the sanitization procedure, toothbrushes were again cultured for the number of CFUs. The collected data of the presanitized and postsanitized CFU count were log transformed to normalize their distributions prior to analysis. Furthermore, log CFU data were compared and analyzed by one-way ANOVA, Tukey's post hoc procedure, and paired t-test for the difference in the mean at P<0.05.

RESULTS

Result showed that after the sanitization procedure, there was a significant (P<0.001) reduction in microbial contamination in both microwave and UV group toothbrushes compared to control group toothbrushes whereas the microbial count in the microwave group was significantly less (P<0.001) compared to the UV group.

CONCLUSIONS

The evidence presented in this study suggests that microwave irradiation is an effective disinfectant agent for bacteria and fungi on toothbrushes.

摘要

背景

牙刷会迅速被不同微生物污染，这可能是感染或再感染的一个原因，尤其是在接受治疗的牙周病患者中。本研究的目的是评估使用家用微波炉和商用紫外线（UV）牙刷消毒器对先前被各种口腔微生物污染的牙刷进行消毒的效果。

材料与方法

30名男性牙科毕业生被随机分为对照组或实验组，并收到用于家庭使用的标准化牙刷。每位受试者被指示使用标准化改良巴氏刷牙法刷牙1周，使用后提交给研究者。收集的牙刷进行培养，并分析菌落形成单位（CFU）数量。在下一阶段，再次给每位受试者一套新牙刷，并指示他们再使用1周，并遵循与之前相同的指示。随后，再次收集使用过的牙刷，并通过微波辐射、紫外线辐射进行消毒，或不进行消毒（对照组）。消毒程序后，再次对牙刷进行CFU数量培养。在分析之前，对预消毒和消毒后CFU计数的收集数据进行对数转换，以使其分布标准化。此外，对对数CFU数据进行单因素方差分析、Tukey事后检验和配对t检验，以比较均值差异，P<0.05。