Shi L, Ma Y, Cai Z
Department of Pharmacy, Changhai Hospital, Second Military Medical University, Shanghai, China.
Biomed Chromatogr. 1998 Jan-Feb;12(1):27-30. doi: 10.1002/(SICI)1099-0801(199801/02)12:1<27::AID-BMC715>3.0.CO;2-E.
An accurate RP-HPLC method for the quantitative determination of two water-soluble biologically active compounds, salidroside (p-hydroxyphenethyl-beta-D-glucoside) and specnuezhenide in the fruits of Ligustrum lucidum Ait (a crude drug in Chinese traditional medicine) was developed. The reversed-phase column was Nova-Pak C18, saturated with water before the injection of the samples and eluted with a mobile phase of methanol:water (4:6, v/v). The detection wavelength was 230 nm. The recoveries of the two compounds were 96.1 and 97.0%, respectively. The contents of salidroside and specnuezhenide in the fruits of Ligustrum lucidum Ait and two traditional Chinese patent medicines in which this crude drug was an important component, Erzhi Pills and Anshenbuxin Pills, were determined.
建立了一种准确的反相高效液相色谱法，用于定量测定女贞子（一种中药原料药）果实中的两种水溶性生物活性化合物红景天苷（对羟基苯乙基-β-D-葡萄糖苷）和女贞苷。反相柱为Nova-Pak C18，进样前用水饱和，用甲醇:水（4:6，v/v）的流动相洗脱。检测波长为230nm。两种化合物的回收率分别为96.1%和97.0%。测定了女贞子果实以及以该原料药为重要成分的两种中成药（二至丸和安神补心丸）中红景天苷和女贞苷的含量。
