阿根廷HIV阳性血清对HIV-1 V3 A至H亚型肽的血清反应性。

Seroreactivity to HIV-1 V3 subtypes A to H peptides of Argentinian HIV-positive sera.

作者信息

Devito C, Levi M, Hinkula J, Fernandez Medina R D, Libonatti O, Wigzell H

机构信息

Department of Microbiology, University of Buenos Aires School of Medicine, Argentina.

出版信息

J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Feb 1;17(2):156-9. doi: 10.1097/00042560-199802010-00009.

DOI:10.1097/00042560-199802010-00009

PMID:9473017

Abstract

Serologic assays could be useful for determining circulating subtypes in different geographic regions. A total of 175 serum samples from the same number of Argentinian HIV-infected patients from Buenos Aires and Rosario were tested against a panel of peptides representing V3 consensus subtypes A through H. A V3 peptide enzyme immunoassay was used for screening the sera. Most sera were reactive with peptides representing subtypes B (58.28%), F (13.14%), and A (8.57%). Cross-reactivity between the remainder of the peptides was observed. Genotypes of eight patients from Rosario were determined and compared with serotyping. Results showed that seven of eight genotyped patients reacted with their respective consensus B peptide and one reacted with consensus B and F. V3 peptide serology proved to be useful for determining HIV-1 clades circulating in Argentina.

摘要

血清学检测对于确定不同地理区域的循环亚型可能有用。对来自布宜诺斯艾利斯和罗萨里奥的175名阿根廷HIV感染患者的相同数量的血清样本进行检测，检测对象为一组代表V3共识亚型A至H的肽段。采用V3肽酶免疫测定法对血清进行筛查。大多数血清与代表B亚型（58.28%）、F亚型（13.14%）和A亚型（8.57%）的肽段发生反应。观察到其余肽段之间存在交叉反应。确定了来自罗萨里奥的8名患者的基因型，并与血清分型结果进行比较。结果显示，8名进行基因分型的患者中有7名与其各自的B亚型共识肽发生反应，1名与B亚型和F亚型共识肽发生反应。V3肽血清学被证明对于确定在阿根廷流行的HIV-1进化枝是有用的。