Cheingsong-Popov R, Osmanov S, Pau C P, Schochetman G, Barin F, Holmes H, Francis G, Ruppach H, Dietrich U, Lister S, Weber J
Department of Genito-Urinary Medicine and Communicable Diseases, Imperial College School of Medicine at St Mary's, London, United Kingdom.
AIDS Res Hum Retroviruses. 1998 Mar 1;14(4):311-8. doi: 10.1089/aid.1998.14.311.
V3 serotyping refers to a system based on binding of antibody in patient sera to V3-loop peptides derived from HIV-1 env genetic subtypes. The V3x serotype represents reactivity of serum from an HIV-1-infected patient (regardless of viral genetic subtype), which reacts preferentially to a V3 peptide derived from the X subtype sequence. We have classified HIV-1 serotypes, determined the relationship between the HIV-1 V3 serotypes and viral genetic subtypes in a large study (n = 125), and evaluated the performance of three different V3 peptide-binding assays. Seven HIV-1 V3 serotypes were identified: A, B, B-Br, B-Th, C, D, and E. Serotypes B-Br and B-Th represent sera that react specifically to peptides derived from Brazilian B (B-Br, GWGR) and Thai B (B-Th, GPGQ) strains. The HIV-1 V3 B, C, and E serotypes correlated closely with their viral env genetic subtypes; 19-26 of 32 B sera (59-79%), 3-4 of 4 C sera (75-100%), and 19-22 of 23 E sera (83-96%) were identified as serotypes B, C, and E, respectively. In contrast, two major V3 serotypes were classified in A sera: A (14-18 of 36 [40-50%]) and C (12-19 of 36 [33-54%]). Similarly, two major V3 serotypes were classified in D sera: B (6-10 of 20 [30-50%]) and D (9-12 of 20 [45-60%]). Serotyping of subtype E sera showed the best concordance with genetic subtypes by all assays. Overall, HIV-1 V3 serotyping produced consistent results among three laboratories. However, HIV-1 V3 serotypes do not distinguish all HIV-1 genetic subtypes. The relative biological significance of the V3 serotypes remains to be elucidated.
V3血清分型是指一种基于患者血清中的抗体与源自HIV-1 env基因亚型的V3环肽结合的系统。V3x血清型代表来自HIV-1感染患者的血清反应性（无论病毒基因亚型如何），其优先与源自X亚型序列的V3肽发生反应。我们在一项大型研究（n = 125）中对HIV-1血清型进行了分类，确定了HIV-1 V3血清型与病毒基因亚型之间的关系，并评估了三种不同的V3肽结合检测方法的性能。鉴定出七种HIV-1 V3血清型：A、B、B-Br、B-Th、C、D和E。血清型B-Br和B-Th代表分别对源自巴西B（B-Br，GWGR）和泰国B（B-Th，GPGQ）毒株的肽具有特异性反应的血清。HIV-1 V3 B、C和E血清型与其病毒env基因亚型密切相关；32份B血清中有19 - 26份（59 - 79%）、4份C血清中有3 - 4份（75 - 100%）、23份E血清中有19 - 22份（83 - 96%）分别被鉴定为血清型B、C和E。相比之下，A血清中分为两种主要的V3血清型：A（36份中的14 - 18份[40 - 50%]）和C（36份中的12 - 19份[33 - 54%]）。同样，D血清中分为两种主要的V3血清型：B（20份中的6 - 10份[30 - 50%]）和D（20份中的9 - 12份[45 - 60%]）。通过所有检测方法，E亚型血清的血清分型与基因亚型的一致性最佳。总体而言，HIV-1 V3血清分型在三个实验室之间产生了一致的结果。然而，HIV-1 V3血清型并不能区分所有的HIV-1基因亚型。V3血清型的相对生物学意义仍有待阐明。
