Lukashov V V, Cornelissen M T, Goudsmit J, Papuashvilli M N, Rytik P G, Khaitov R M, Karamov E V, de Wolf F
Human Retrovirus Laboratory, University of Amsterdam, The Netherlands.
AIDS. 1995 May;9(5):435-9.
To investigate genotypes and serotypes of HIV-1 variants in Russia, Byelorussia and Lithuania.
Sera from 20 HIV-1-infected individuals were tested in an enzyme-linked immunosorbent assay (ELISA) with 19 V3 synthetic peptides, and serum HIV-1 V3 RNA was amplified and sequenced.
Sequence comparison of the envelope V3 region among specimens tested revealed a 2-29% range nucleotide divergence, with a mean of 19%. Phylogenetic analysis from the homosexual men were shown to belong to subtype B, and all of the heterosexually infected individuals to subtype C. Sequences from the parenterally infected individuals were more heterogeneous. IOn the peptide ELISA three reactivity patterns were found. Serum samples from six out of seven homosexual men showed reactivity to peptides p108 or p110 representing V3 amino-acid sequences found in US/West European HIV-1 isolates. Serum samples from six of seven individuals who had acquired HIV-1 through heterosexual contacts were reactive to peptide p169. Four out of six parenterally infected patients had peak reactivity to p168.
Distinct HIV-1 variants were found in Russia, Byelorussia and Lithuania, which were introduced simultaneously in the mid-1980s. This diversity was shown to be associated with the route of transmission. Homosexual men appeared to be infected with subtype B and heterosexually infected individuals with subtype C HIV-1 variants. HIV-1 subtypes A, C, D and G were found among parenterally infected individuals.
研究俄罗斯、白俄罗斯和立陶宛的HIV-1变异株的基因型和血清型。
用19种V3合成肽通过酶联免疫吸附测定（ELISA）检测20例HIV-1感染个体的血清，并对血清HIV-1 V3 RNA进行扩增和测序。
对检测标本的包膜V3区进行序列比较，发现核苷酸差异范围为2% - 29%，平均为19%。系统发育分析显示，同性恋男性感染的毒株属于B亚型，所有异性感染个体的毒株属于C亚型。经静脉感染个体的序列更具异质性。在肽ELISA中发现了三种反应模式。7名同性恋男性中的6名血清样本对代表美国/西欧HIV-1分离株中发现的V3氨基酸序列的肽p108或p110有反应。7名通过异性接触感染HIV-1的个体中有6名的血清样本对肽p169有反应。6名经静脉感染的患者中有4名对p168的反应峰值最高。
在俄罗斯、白俄罗斯和立陶宛发现了不同的HIV-1变异株，这些变异株于20世纪80年代中期同时传入。这种多样性与传播途径有关。同性恋男性似乎感染的是B亚型，异性感染个体感染的是C亚型HIV-1变异株。在经静脉感染的个体中发现了HIV-1 A、C、D和G亚型。
