Reeves F C, Fredericks W J, Rauscher F J, Lillycrop K A
Department of Biochemistry, School of Biological Sciences, University of Southampton, Bassett Crescent East, UK.
FEBS Lett. 1998 Jan 23;422(1):118-22. doi: 10.1016/s0014-5793(97)01598-6.
Mutations in the murine Pax-3 gene lead to a range of developmental abnormalities including deficiencies in sensory and sympathetic neurones. We have investigated Pax-3 expression during neuronal differentiation and show levels of Pax-3 DNA binding decrease upon cell cycle arrest and morphological differentiation. The fall in Pax-3 DNA binding occurs within 1 h of the induction of differentiation and is mediated in part by a decrease in Pax-3 mRNA. This decrease in Pax-3 binding activity precedes any changes in cell proliferation or morphology, suggesting that the downregulation of this transcription factor may be an important prerequisite for the differentiation of neuronal progenitor cells.
小鼠Pax-3基因的突变会导致一系列发育异常，包括感觉神经元和交感神经元的缺陷。我们研究了神经元分化过程中Pax-3的表达，发现细胞周期停滞和形态分化时Pax-3 DNA结合水平降低。Pax-3 DNA结合的下降在诱导分化后1小时内发生，部分是由Pax-3 mRNA的减少介导的。Pax-3结合活性的这种下降发生在细胞增殖或形态的任何变化之前，这表明该转录因子的下调可能是神经元祖细胞分化的重要先决条件。
