van Beurden M, ten Kate F W, Tjong-A-Hung S P, de Craen A J, van der Vange N, Lammes F B, ter Schegget J
Department of Obstetrics and Gynecology, Academic Medical Centre, Amsterdam, The Netherlands.
Int J Gynecol Pathol. 1998 Jan;17(1):12-6. doi: 10.1097/00004347-199801000-00003.
Human papillomavirus (HPV) DNA in vulvar intraepithelial neoplasia (VIN) is associated with multifocality of VIN III and with multicentricity of other neoplastic squamous lesions in the cervix and vagina. The aim of this study was to establish the prevalence and type of HPV DNA in the lesions of vaginal intraepithelial neoplasia (VaIN) and cervical intraepithelial neoplasia (CIN) in patients with VIN III using the polymerase chain reaction (PCR). HPV DNA detection and histologic analysis were performed on alternating sections of paraffin-embedded biopsies of concomitant CIN and VaIN in 27 patients with VIN III. PCR was performed with consensus primers and HPV typing was performed by direct sequencing of the PCR amplimers. HPV DNA was detected in all VIN III lesions (93% contained HPV-16 DNA); in 96% of the CIN lesions (73% contained HPV-16 DNA); and in all VaIN lesions (75% contained HPV-16 DNA). The HPV type was not the same in 22% of the different lesions of VIN, CIN, and VaIN, even if the biopsies were taken at the same time.
外阴上皮内瘤变（VIN）中的人乳头瘤病毒（HPV）DNA与VIN III的多灶性以及宫颈和阴道中其他肿瘤性鳞状病变的多中心性相关。本研究的目的是使用聚合酶链反应（PCR）确定III级VIN患者阴道上皮内瘤变（VaIN）和宫颈上皮内瘤变（CIN）病变中HPV DNA的患病率和类型。对27例III级VIN患者的CIN和VaIN石蜡包埋活检交替切片进行HPV DNA检测和组织学分析。用通用引物进行PCR，通过对PCR扩增产物直接测序进行HPV分型。在所有VIN III病变中均检测到HPV DNA（93%含有HPV-16 DNA）；在96%的CIN病变中（73%含有HPV-16 DNA）；在所有VaIN病变中（75%含有HPV-16 DNA）。即使活检是同时进行的，在22%的VIN、CIN和VaIN不同病变中HPV类型也不相同。
