Andersson S, Alemi M, Rylander E, Strand A, Larsson B, Sällström J, Wilander E
Department of Obstetrics and Gynaecology, Danderyd Hospital, Karolinska Institutet, Stockholm, Sweden.
Br J Cancer. 2000 Aug;83(3):307-10. doi: 10.1054/bjoc.2000.1247.
A previous Swedish study revealed that both prototype and variant HPV16 E6 oncoprotein, occur in about equal numbers in high-grade cervical intraepithelial neoplasia (HCIN), whereas variant HPV16 predominates in invasive cervical squamous carcinoma. Most of the malignant HPV16 variants contain a common mutation, L83V, in the E6 oncoprotein. In the present investigation, 28 HPV16 positive, invasive cervical adenocarcinomas were collected from a total number of 131 adenocarcinomas. These HPV16-positive cases were evaluated with analysis of the E6 gene, using a recently described PCR-SSCP method for identification of the specific mutation (L83V) in the E6 gene. The results obtained were correlated to findings in 103 preinvasive, HCIN, and 31 invasive cervical squamous carcinomas also infected with HPV16. The HPV16 E6 variant L83V was present in 40% of the HCIN lesions, in 54% of the invasive adenocarcinomas, in comparison to 81% of the invasive squamous carcinomas. The difference between HCIN and squamous carcinomas was statistically significant, P < 0.001, whereas the difference between HCIN and invasive adenocarcinomas was not statistically significant, P = 0.604. Prototype HPV16 and its E6 variant L83V are both prevalent in preinvasive and invasive cervical lesions in Swedish women. However, the obvious predominance of HPV16 variant in squamous carcinomas was not seen in adenocarcinomas. A single amino-acid shift in the HPV16 E6 gene appears to result in a different transforming potential in squamous and glandular cervical lesions.
瑞典此前的一项研究显示，在高级别宫颈上皮内瘤变（HCIN）中，HPV16 E6癌蛋白原型和变体的数量大致相等，而在宫颈浸润性鳞状细胞癌中，HPV16变体占主导地位。大多数恶性HPV16变体在E6癌蛋白中含有一个常见突变L83V。在本研究中，从131例腺癌中收集了28例HPV16阳性的宫颈浸润性腺癌。使用最近描述的PCR-SSCP方法对这些HPV16阳性病例的E6基因进行分析，以鉴定E6基因中的特定突变（L83V）。将所得结果与103例HPV16感染的宫颈上皮内瘤变（preinvasive）和31例宫颈浸润性鳞状细胞癌的结果进行关联分析。HPV16 E6变体L83V在40%的HCIN病变、54%的浸润性腺癌中出现，相比之下，在81%的浸润性鳞状细胞癌中出现。HCIN与鳞状细胞癌之间的差异具有统计学意义，P<0.001，而HCIN与浸润性腺癌之间的差异无统计学意义，P = 0.604。HPV16原型及其E6变体L83V在瑞典女性的宫颈上皮内瘤变和浸润性病变中均普遍存在。然而，HPV16变体在鳞状细胞癌中明显占主导的情况在腺癌中并未出现。HPV16 E6基因中的单个氨基酸变化似乎导致了宫颈鳞状和腺性病变中不同的转化潜能。
