Mittal K, Demopoulos R I, Tata M
Department of Pathology, New York University Medical Center and Kaplan Cancer Center, New York, USA.
Int J Gynecol Pathol. 1998 Jan;17(1):24-8. doi: 10.1097/00004347-199801000-00005.
Cervical condylomas associated with HPV types 6/11 rarely progress to dysplasia; this progression is more commonly seen in cervical condylomas with HPV types 16/18/31/33/35. This investigation was undertaken to determine if more frequent atypical mitotic figures (MFs) and higher proliferative activity are seen in high-risk condylomas. HPV types present in cervical condylomas were determined by in situ hybridization with biotinylated probes. The cases were also stained immunohistochemically for MIB1. The percentage staining of basal, parabasal, and suprabasal cells was determined by counting 100 cells in the most intensely stained areas. MFs and atypical MFs were counted per 10 high-power-fields (HPFs). Condylomas with HPV 6/11 showed higher MIB1 expression in the basal layer than condylomas with HPV 16/18 and 31/33/35 (p = 0.013). Atypical MFs were seen more frequently in condylomas with HPV types 16/18/31/33/35 (p = 0.02). Differences in mitotic activity and in MIB1 expression in parabasal and suprabasal layers did not reach statistical significance. The presence of atypical MFs may make a greater contribution than increased proliferative activity to progression to dysplasia in cervical condylomas.
与6/11型人乳头瘤病毒（HPV）相关的宫颈湿疣很少进展为发育异常；这种进展在与16/18/31/33/35型HPV相关的宫颈湿疣中更为常见。本研究旨在确定在高危湿疣中是否可见更频繁的非典型有丝分裂象（MFs）和更高的增殖活性。通过用生物素化探针进行原位杂交来确定宫颈湿疣中存在的HPV类型。这些病例还进行了MIB1免疫组织化学染色。通过在染色最强烈的区域计数100个细胞来确定基底细胞、副基底细胞和基底上层细胞的染色百分比。每10个高倍视野（HPFs）计数MFs和非典型MFs。与16/18型和31/33/35型HPV相关的湿疣相比，6/11型HPV相关的湿疣在基底层显示出更高的MIB1表达（p = 0.013）。非典型MFs在16/18/31/33/35型HPV相关的湿疣中更常见（p = 0.02）。副基底细胞层和基底上层细胞层的有丝分裂活性和MIB1表达差异未达到统计学意义。非典型MFs的存在可能比增殖活性增加对宫颈湿疣进展为发育异常的贡献更大。
