Sireci G, Champagne E, Fourniè J J, Dieli F, Salerno A
Institute of General Pathology, University of Palermo, Italy.
Hum Immunol. 1997 Dec;58(2):70-82. doi: 10.1016/s0198-8859(97)00211-5.
This paper examines functional properties of human Vgamma9/Vdelta2 T cell lines and clones generated by in vitro culture with synthetic and natural (mycobacterial) phosphoantigenic molecules. It confirms the broad reactivity of Vgamma9/Vdelta2 T cell lines and clones toward phosphoantigens. Optimal recognition of phosphoantigens by Vgamma9/Vdelta2 T cells required accessory cells to occur, but did not require specialized antigen presenting cells. However, species origin of the APC was irrelevant as proliferation of Vgamma9/Vdelta2 T cells occurred in the presence of syngeneic, allogeneic or xenogeneic APC and was not restricted to APC of particular tissue origin. Moreover antigen uptake and processing was not required for recognition by Vgamma9/ Vdelta2 cells, as evidenced by the ability of fixed APCs to present phosphoantigens. Similarly, the expression of classical MHC class I and class II molecules was not required for phosphoantigen recognition by gammadelta T cells. However, gammadelta T cell clones responded to stimulation by several cytokines including IL-12, IFNgamma and TNFalpha. Finally, Vgamma9/Vdelta2 T cell clones preferentially produced both IFN-gamma and IL-4 in response to PHA or TUBAg stimulation, revealing that a Th0 pattern of cytokine production is frequent among these cells.
本文研究了通过与合成及天然（分枝杆菌）磷酸抗原分子进行体外培养所产生的人Vγ9/Vδ2 T细胞系及克隆的功能特性。该研究证实了Vγ9/Vδ2 T细胞系及克隆对磷酸抗原具有广泛的反应性。Vγ9/Vδ2 T细胞对磷酸抗原的最佳识别需要辅助细胞的存在，但并不需要专门的抗原呈递细胞。然而，抗原呈递细胞的物种来源无关紧要，因为Vγ9/Vδ2 T细胞在同基因、异基因或异种抗原呈递细胞存在的情况下均可增殖，且并不局限于特定组织来源的抗原呈递细胞。此外，固定的抗原呈递细胞能够呈递磷酸抗原，这表明Vγ9/Vδ2细胞识别抗原时不需要抗原摄取和加工。同样，γδ T细胞识别磷酸抗原并不需要经典的MHC I类和II类分子的表达。然而，γδ T细胞克隆对包括IL-12、IFNγ和TNFα在内的多种细胞因子的刺激均有反应。最后，Vγ9/Vδ2 T细胞克隆在受到PHA或TUBAg刺激时优先产生IFN-γ和IL-4，这表明这些细胞中细胞因子产生的Th0模式较为常见。
