King J C, Liu E, Ronsheim P M, Slonimski M, Rubin B S
Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Biol Reprod. 1998 Feb;58(2):316-22. doi: 10.1095/biolreprod58.2.316.
The induction of Fos protein was examined within LHRH neurons of guinea pigs; the aim was to delineate relationships between subgroups of LHRH neurons during an LH surge in a laboratory rodent in which the distribution of LHRH neurons and the presence of a true luteal phase in the reproductive cycle resemble those in primates. Approximately one third of the forebrain population of LHRH neurons was examined in ovariectomized steroid-treated guinea pigs killed either before or during a steroid-induced LH surge. LHRH/Fos double-labeled neurons were more abundant in surging compared to presurge guinea pigs (p = 0.008) and were most abundant within the preoptic area and anterior hypothalamus. Nonetheless, double-labeled LHRH/Fos neurons were observed throughout the remainder of the population of LHRH neurons in surging guinea pigs. A relative loss of LHRH reaction product was detected by image analysis in the LHRH terminals in the median eminence of surging guinea pigs, consistent with augmented LHRH release. Thus, there appears to be a coordinated increase in Fos expression in subgroups of LHRH neurons, more pronounced in rostral, as compared to caudal, regions in guinea pigs killed after the peak of the steroid-induced LH surge.
研究了豚鼠促性腺激素释放激素（LHRH）神经元中Fos蛋白的诱导情况；目的是在一种实验性啮齿动物的促黄体生成素（LH）峰期间，描绘LHRH神经元亚群之间的关系，该啮齿动物的LHRH神经元分布和生殖周期中真正黄体期的存在与灵长类动物相似。在类固醇处理的去卵巢豚鼠中，于类固醇诱导的LH峰之前或期间处死，检查了约三分之一前脑LHRH神经元群体。与LH峰前的豚鼠相比，LH峰时LHRH/Fos双标记神经元更为丰富（p = 0.008），且在视前区和下丘脑前部最为丰富。尽管如此，在LH峰时的豚鼠中，在LHRH神经元群体的其余部分也观察到了双标记的LHRH/Fos神经元。通过图像分析在LH峰时的豚鼠正中隆起的LHRH终末中检测到LHRH反应产物相对减少，这与LHRH释放增加一致。因此，在类固醇诱导的LH峰达到峰值后处死的豚鼠中，与尾侧区域相比，LHRH神经元亚群中Fos表达似乎有协调性增加，在 Rostral区域更为明显。
