King J C, Ronsheim P, Liu E, Powers L, Slonimski M, Rubin B S
Tufts University School of Medicine, Boston, Massachusetts 02111, USA.
Biol Reprod. 1998 Feb;58(2):323-9. doi: 10.1095/biolreprod58.2.323.
LHRH neurons in guinea pigs, as in primates and other non-rodent species, are broadly distributed in the basal forebrain. In this study, knife cuts were made in the anterior hypothalamus, effectively separating more caudally positioned hypothalamic LHRH neurons from those in rostral preoptic areas. Guinea pigs with knife cuts displayed an LH surge in response to steroid administration. There was no significant difference in the number of LHRH neurons that expressed Fos in conjunction with an LH surge, although fewer total LHRH neurons were detected in the forebrain of knife-cut versus sham-cut animals. Knife-cut animals displayed a larger percentage of LHRH/Fos neurons in one region of the caudal hypothalamus than sham-cut animals. The area and perimeter of the LHRH reaction product within the cytoplasm of LHRH/Fos neurons were smaller than those of single-labeled LHRH neurons in sham-cut animals and in the caudal hypothalamus, but not the rostral preoptic area, of knife-cut animals. We conclude that caudal hypothalamic LHRH neurons separated from rostral preoptic regions are capable of sustaining an LH surge in guinea pigs. This finding is important, as LHRH neurons are present in the caudal hypothalamus, as well as in preoptic areas, of a large number of mammalian species, including humans.
与灵长类动物和其他非啮齿类动物一样，豚鼠的促性腺激素释放激素（LHRH）神经元广泛分布于基底前脑。在本研究中，在前下丘脑进行刀切，有效地将更靠尾侧的下丘脑LHRH神经元与视前区前部的神经元分开。接受刀切的豚鼠在给予类固醇后出现促黄体生成素（LH）高峰。尽管与假手术组动物相比，刀切动物前脑检测到的LHRH神经元总数较少，但在LH高峰时表达Fos的LHRH神经元数量没有显著差异。与假手术组动物相比，刀切动物在下丘脑尾侧的一个区域中，LHRH/Fos神经元的比例更大。LHRH/Fos神经元细胞质内LHRH反应产物的面积和周长小于假手术组动物以及刀切动物下丘脑尾侧（而非视前区前部）单标记LHRH神经元的面积和周长。我们得出结论，与视前区前部分离的下丘脑尾侧LHRH神经元能够维持豚鼠的LH高峰。这一发现很重要，因为包括人类在内的大量哺乳动物物种的下丘脑尾侧以及视前区都存在LHRH神经元。
