Faxén M, Nasiell J, Blanck A, Nisell H, Lunell N O
Department of Obstetrics and Gynecology, Karolinska Institute, Huddinge University Hospital, Sweden.
Am J Perinatol. 1998 Jan;15(1):9-13. doi: 10.1055/s-2007-993890.
Placental pathology in preeclampsia (PE) and intrauterine growth retardation (IUGR) is associated with alterations of the placental epidermal growth factor receptor (EGFR). It is encoded by a single gene, which gives rise to two different full-length transcripts and one putative truncated transcript in the placenta. The aim was to investigate if the placental mRNA expression pattern of EGFR differs between women with PE and/or IUGR and normal pregnancies. Tissue samples from placentas were obtained immediately after delivery. Total nucleic acids were prepared and mRNA levels of EGFR transcripts were measured by a solution hybridization technique. In the group with IUGR the placental mRNA expression of the two full-length transcripts was found to be significantly lower than in placentas from normal pregnancy, whereas the expression of the truncated transcript was higher. The groups with PE or PE with IUGR had a significantly higher mRNA expression of the truncated transcript, while there were no significant differences in the mRNA expression of the two full-length transcripts. These findings are consistent with an important role for EGFR in the regulation of placental and fetal growth.
子痫前期（PE）和胎儿宫内生长受限（IUGR）中的胎盘病理与胎盘表皮生长因子受体（EGFR）的改变有关。它由单个基因编码，该基因在胎盘中产生两种不同的全长转录本和一种推定的截短转录本。目的是研究患有PE和/或IUGR的女性与正常妊娠女性之间EGFR的胎盘mRNA表达模式是否存在差异。分娩后立即获取胎盘组织样本。制备总核酸，并通过溶液杂交技术测量EGFR转录本的mRNA水平。在IUGR组中，发现两种全长转录本的胎盘mRNA表达明显低于正常妊娠胎盘，而截短转录本的表达较高。PE组或合并IUGR的PE组截短转录本的mRNA表达明显更高，而两种全长转录本的mRNA表达没有显著差异。这些发现与EGFR在调节胎盘和胎儿生长中的重要作用一致。
